Results 21 - 28 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11425 | 3' | -55.2 | NC_003085.1 | + | 27989 | 0.69 | 0.478811 |
Target: 5'- cGC-CGcGCAGgCCgcCCUUGUCGGCg -3' miRNA: 3'- -UGaGUuCGUCgGGaaGGAACAGCCGg -5' |
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11425 | 3' | -55.2 | NC_003085.1 | + | 37793 | 0.69 | 0.478811 |
Target: 5'- aACUCA--CAGCCCcaCCUccUCGGCCa -3' miRNA: 3'- -UGAGUucGUCGGGaaGGAacAGCCGG- -5' |
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11425 | 3' | -55.2 | NC_003085.1 | + | 656 | 0.69 | 0.468538 |
Target: 5'- cGCUCGAGCAccGCCCggaCgucGcCGGCCa -3' miRNA: 3'- -UGAGUUCGU--CGGGaagGaa-CaGCCGG- -5' |
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11425 | 3' | -55.2 | NC_003085.1 | + | 1494 | 0.7 | 0.448338 |
Target: 5'- uGCUCGAcGCGGCCCgUCCggcgcUCGGgCa -3' miRNA: 3'- -UGAGUU-CGUCGGGaAGGaac--AGCCgG- -5' |
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11425 | 3' | -55.2 | NC_003085.1 | + | 47067 | 0.71 | 0.40005 |
Target: 5'- aGCUC-GGCGGUg--UCCaUGUCGGCCa -3' miRNA: 3'- -UGAGuUCGUCGggaAGGaACAGCCGG- -5' |
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11425 | 3' | -55.2 | NC_003085.1 | + | 18483 | 0.71 | 0.390797 |
Target: 5'- aACUC--GCAGCCCccgUCCUcaaggCGGCCa -3' miRNA: 3'- -UGAGuuCGUCGGGa--AGGAaca--GCCGG- -5' |
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11425 | 3' | -55.2 | NC_003085.1 | + | 16240 | 0.73 | 0.283771 |
Target: 5'- gGCUCAuGgAGCCCggCCgagUGccUCGGCCa -3' miRNA: 3'- -UGAGUuCgUCGGGaaGGa--AC--AGCCGG- -5' |
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11425 | 3' | -55.2 | NC_003085.1 | + | 40090 | 1.11 | 0.000562 |
Target: 5'- uACUCAAGCAGCCCUUCCUUGUCGGCCu -3' miRNA: 3'- -UGAGUUCGUCGGGAAGGAACAGCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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