Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11426 | 5' | -57.9 | NC_003085.1 | + | 30050 | 0.66 | 0.52464 |
Target: 5'- aCCAgguGGCGcCGCCCUGGGC-CUugcUGGg -3' miRNA: 3'- aGGU---UCGC-GCGGGACCUGaGGu--ACUg -5' |
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11426 | 5' | -57.9 | NC_003085.1 | + | 41130 | 0.66 | 0.534185 |
Target: 5'- cUCCAcGCGCccgaugaggaaguGCCCucggUGGACUUCguagGUGACg -3' miRNA: 3'- -AGGUuCGCG-------------CGGG----ACCUGAGG----UACUG- -5' |
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11426 | 5' | -57.9 | NC_003085.1 | + | 45494 | 0.66 | 0.535249 |
Target: 5'- -aCAAGCGCGgCCgucuGGCUCCAUccccgggccgcGACa -3' miRNA: 3'- agGUUCGCGCgGGac--CUGAGGUA-----------CUG- -5' |
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11426 | 5' | -57.9 | NC_003085.1 | + | 39718 | 1.1 | 0.000342 |
Target: 5'- uUCCAAGCGCGCCCUGGACUCCAUGACg -3' miRNA: 3'- -AGGUUCGCGCGGGACCUGAGGUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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