Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 11429 | 5' | -52.5 | NC_003085.1 | + | 9522 | 0.66 | 0.793631 |
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Target: 5'- gCGGGUCAUCG-GCucCCugguaguGCACGaCGCGg -3' miRNA: 3'- -GUUCAGUAGCuCGu-GGu------UGUGC-GCGU- -5' |
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| 11429 | 5' | -52.5 | NC_003085.1 | + | 21536 | 0.66 | 0.793631 |
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Target: 5'- gCAAGUCc-CG-GUACCGGaacCACGCGCGg -3' miRNA: 3'- -GUUCAGuaGCuCGUGGUU---GUGCGCGU- -5' |
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| 11429 | 5' | -52.5 | NC_003085.1 | + | 7494 | 0.66 | 0.810494 |
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Target: 5'- aCGGGcguccgCGUCGAGCgguugcaggacaucACCGACgaggACGCGCGg -3' miRNA: 3'- -GUUCa-----GUAGCUCG--------------UGGUUG----UGCGCGU- -5' |
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| 11429 | 5' | -52.5 | NC_003085.1 | + | 46279 | 0.66 | 0.803617 |
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Target: 5'- uGAGUCGUCGAGUGCC--CAUGUcaGCc -3' miRNA: 3'- gUUCAGUAGCUCGUGGuuGUGCG--CGu -5' |
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| 11429 | 5' | -52.5 | NC_003085.1 | + | 13806 | 0.66 | 0.832377 |
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Target: 5'- -cAGUCugcgUGgagguGGCGCCAugACGUGCAg -3' miRNA: 3'- guUCAGua--GC-----UCGUGGUugUGCGCGU- -5' |
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| 11429 | 5' | -52.5 | NC_003085.1 | + | 29667 | 0.66 | 0.832377 |
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Target: 5'- gAAGUCAugUCGGGCGuCCAccuCGCGaCGUg -3' miRNA: 3'- gUUCAGU--AGCUCGU-GGUu--GUGC-GCGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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