Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11429 | 5' | -52.5 | NC_003085.1 | + | 37435 | 0.75 | 0.32433 |
Target: 5'- gAAGUCG-CGcAGCACCGGCAgGUGCGc -3' miRNA: 3'- gUUCAGUaGC-UCGUGGUUGUgCGCGU- -5' |
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11429 | 5' | -52.5 | NC_003085.1 | + | 47707 | 0.76 | 0.300431 |
Target: 5'- gCGAGUCGUCGAcugcuGCGCCugcuGACGgGCGCGg -3' miRNA: 3'- -GUUCAGUAGCU-----CGUGG----UUGUgCGCGU- -5' |
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11429 | 5' | -52.5 | NC_003085.1 | + | 831 | 0.76 | 0.263666 |
Target: 5'- gCAGGUCGUCGAGCGCgucucuCACGuCGCGc -3' miRNA: 3'- -GUUCAGUAGCUCGUGguu---GUGC-GCGU- -5' |
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11429 | 5' | -52.5 | NC_003085.1 | + | 38153 | 0.78 | 0.224467 |
Target: 5'- -cAGUCcUCGuccacaAGCGCCAGCGCGCGCu -3' miRNA: 3'- guUCAGuAGC------UCGUGGUUGUGCGCGu -5' |
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11429 | 5' | -52.5 | NC_003085.1 | + | 9681 | 0.8 | 0.165449 |
Target: 5'- ----aCGUCGAGCACCGGCugGCGUc -3' miRNA: 3'- guucaGUAGCUCGUGGUUGugCGCGu -5' |
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11429 | 5' | -52.5 | NC_003085.1 | + | 38513 | 1.08 | 0.001689 |
Target: 5'- cCAAGUCAUCGAGCACCAACACGCGCAc -3' miRNA: 3'- -GUUCAGUAGCUCGUGGUUGUGCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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