Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11432 | 5' | -55.4 | NC_003085.1 | + | 40603 | 0.66 | 0.704079 |
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Target: 5'- aCGUGGAUGCcgaugcacGUCAUggccGCCCCUCc- -3' miRNA: 3'- aGCAUCUGCGca------CAGUA----CGGGGAGcu -5' |
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| 11432 | 5' | -55.4 | NC_003085.1 | + | 11778 | 0.66 | 0.693249 |
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Target: 5'- aCGgcGGCgccgcgcuccggGCGUGUCGgcUGCCCCaUCGc -3' miRNA: 3'- aGCauCUG------------CGCACAGU--ACGGGG-AGCu -5' |
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| 11432 | 5' | -55.4 | NC_003085.1 | + | 11123 | 0.66 | 0.682361 |
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Target: 5'- uUCGUugGGGCGCGcGUC--GUCUCUCGGa -3' miRNA: 3'- -AGCA--UCUGCGCaCAGuaCGGGGAGCU- -5' |
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| 11432 | 5' | -55.4 | NC_003085.1 | + | 17915 | 0.66 | 0.660451 |
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Target: 5'- gUCGUAGACGgGUGcC-UGCgCCCagcgCGGc -3' miRNA: 3'- -AGCAUCUGCgCACaGuACG-GGGa---GCU- -5' |
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| 11432 | 5' | -55.4 | NC_003085.1 | + | 26717 | 0.67 | 0.627413 |
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Target: 5'- gUCGUcGGCGCGggUGUCGgagccgagUGCUUCUCGGc -3' miRNA: 3'- -AGCAuCUGCGC--ACAGU--------ACGGGGAGCU- -5' |
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| 11432 | 5' | -55.4 | NC_003085.1 | + | 7484 | 0.67 | 0.613092 |
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Target: 5'- aCGgcGAgGCGUGUCAcugggaagcgcaggUGCgUCUCGGu -3' miRNA: 3'- aGCauCUgCGCACAGU--------------ACGgGGAGCU- -5' |
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| 11432 | 5' | -55.4 | NC_003085.1 | + | 35110 | 0.7 | 0.447471 |
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Target: 5'- cCGUugAGugGCaG-GUCcgGCCCCUCGu -3' miRNA: 3'- aGCA--UCugCG-CaCAGuaCGGGGAGCu -5' |
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| 11432 | 5' | -55.4 | NC_003085.1 | + | 41874 | 0.7 | 0.436741 |
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Target: 5'- ---cGGACGCGUGUCGgaUGCguacgugaugaacCCCUCGGc -3' miRNA: 3'- agcaUCUGCGCACAGU--ACG-------------GGGAGCU- -5' |
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| 11432 | 5' | -55.4 | NC_003085.1 | + | 37713 | 1.09 | 0.000917 |
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Target: 5'- gUCGUAGACGCGUGUCAUGCCCCUCGAc -3' miRNA: 3'- -AGCAUCUGCGCACAGUACGGGGAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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