Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11435 | 3' | -52.9 | NC_003085.1 | + | 8111 | 0.66 | 0.782101 |
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Target: 5'- ----cGCGAagUUGGGCGCUgaUGACGu -3' miRNA: 3'- uugaaCGUU--GACCCGCGAgaACUGCu -5' |
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| 11435 | 3' | -52.9 | NC_003085.1 | + | 41059 | 0.66 | 0.771644 |
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Target: 5'- gGGCUgcuacGCGACUGGGgGCUgccGAUGAg -3' miRNA: 3'- -UUGAa----CGUUGACCCgCGAgaaCUGCU- -5' |
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| 11435 | 3' | -52.9 | NC_003085.1 | + | 2778 | 0.66 | 0.750275 |
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Target: 5'- aAGCagGCAGCgGcGGCGCUCcaGGCGGc -3' miRNA: 3'- -UUGaaCGUUGaC-CCGCGAGaaCUGCU- -5' |
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| 11435 | 3' | -52.9 | NC_003085.1 | + | 30662 | 0.67 | 0.706067 |
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Target: 5'- cGC-UGCGGCaGGGCGCUCU--GCGc -3' miRNA: 3'- uUGaACGUUGaCCCGCGAGAacUGCu -5' |
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| 11435 | 3' | -52.9 | NC_003085.1 | + | 5628 | 0.67 | 0.683439 |
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Target: 5'- gGACggGCuGCUGGaGCGCUCgcccugUGACu- -3' miRNA: 3'- -UUGaaCGuUGACC-CGCGAGa-----ACUGcu -5' |
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| 11435 | 3' | -52.9 | NC_003085.1 | + | 36307 | 1.07 | 0.001576 |
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Target: 5'- gAACUUGCAACUGGGCGCUCUUGACGAa -3' miRNA: 3'- -UUGAACGUUGACCCGCGAGAACUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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