Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11441 | 3' | -55.5 | NC_003085.1 | + | 38798 | 0.66 | 0.623397 |
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Target: 5'- cGUCGCUGCGCUgUCGcuCAGccAGGcGUGUg -3' miRNA: 3'- -CAGCGACGUGA-AGCccGUU--UCC-CACA- -5' |
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| 11441 | 3' | -55.5 | NC_003085.1 | + | 45256 | 0.66 | 0.612186 |
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Target: 5'- -cCGCgUGCGCUUCGGuGguGAGGuUGg -3' miRNA: 3'- caGCG-ACGUGAAGCC-CguUUCCcACa -5' |
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| 11441 | 3' | -55.5 | NC_003085.1 | + | 7781 | 0.66 | 0.612186 |
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Target: 5'- cGUCGCggcgaGCGCaUUCGcGGCccaacAGGGUGg -3' miRNA: 3'- -CAGCGa----CGUG-AAGC-CCGuu---UCCCACa -5' |
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| 11441 | 3' | -55.5 | NC_003085.1 | + | 36570 | 0.67 | 0.586483 |
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Target: 5'- gGUCGCcauUGCACgggaggcuucgagggCGGGCAc-GGGUGg -3' miRNA: 3'- -CAGCG---ACGUGaa-------------GCCCGUuuCCCACa -5' |
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| 11441 | 3' | -55.5 | NC_003085.1 | + | 9560 | 0.67 | 0.578697 |
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Target: 5'- -aCGCUGgGCUUCu-GCAuGGGGUGUc -3' miRNA: 3'- caGCGACgUGAAGccCGUuUCCCACA- -5' |
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| 11441 | 3' | -55.5 | NC_003085.1 | + | 42586 | 0.69 | 0.481544 |
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Target: 5'- aUCGggGCuauCUUCGGGCGGAcGGGUaGUg -3' miRNA: 3'- cAGCgaCGu--GAAGCCCGUUU-CCCA-CA- -5' |
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| 11441 | 3' | -55.5 | NC_003085.1 | + | 40539 | 0.71 | 0.331846 |
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Target: 5'- -gCGCUGCACUUCGGaggcgucgaggcGCAAGGccuuGGUGg -3' miRNA: 3'- caGCGACGUGAAGCC------------CGUUUC----CCACa -5' |
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| 11441 | 3' | -55.5 | NC_003085.1 | + | 32926 | 0.98 | 0.004407 |
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Target: 5'- aGUCGCUGC-CUUCGGGCAAAGGGUGUc -3' miRNA: 3'- -CAGCGACGuGAAGCCCGUUUCCCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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