Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11445 | 3' | -54.3 | NC_003085.1 | + | 23186 | 0.66 | 0.723361 |
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Target: 5'- cCUGCGugGCAaacGUCCAUUGcuGcUGCAg -3' miRNA: 3'- aGAUGCugCGU---CAGGUGACc-CaACGU- -5' |
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| 11445 | 3' | -54.3 | NC_003085.1 | + | 38175 | 0.68 | 0.623397 |
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Target: 5'- --cGCGGCGCAGguguUCCcCUGGGcugGCGg -3' miRNA: 3'- agaUGCUGCGUC----AGGuGACCCaa-CGU- -5' |
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| 11445 | 3' | -54.3 | NC_003085.1 | + | 9547 | 0.69 | 0.567614 |
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Target: 5'- --cACGACGCGGccgaCGCUGGGcuucUGCAu -3' miRNA: 3'- agaUGCUGCGUCag--GUGACCCa---ACGU- -5' |
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| 11445 | 3' | -54.3 | NC_003085.1 | + | 180 | 0.69 | 0.556585 |
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Target: 5'- --cGCGACGCAGcagCCACaggGGGUgGCc -3' miRNA: 3'- agaUGCUGCGUCa--GGUGa--CCCAaCGu -5' |
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| 11445 | 3' | -54.3 | NC_003085.1 | + | 36496 | 0.69 | 0.534723 |
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Target: 5'- -gUugGACGCAGUCCc---GGUUGCu -3' miRNA: 3'- agAugCUGCGUCAGGugacCCAACGu -5' |
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| 11445 | 3' | -54.3 | NC_003085.1 | + | 27348 | 0.71 | 0.450892 |
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Target: 5'- gCUGCGGCGCcgGGcUCUGCUGGGcgGCc -3' miRNA: 3'- aGAUGCUGCG--UC-AGGUGACCCaaCGu -5' |
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| 11445 | 3' | -54.3 | NC_003085.1 | + | 35779 | 0.72 | 0.396698 |
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Target: 5'- --cGCGGCGCGacggccgguguccuuGUCCAUgGGGUUGCGc -3' miRNA: 3'- agaUGCUGCGU---------------CAGGUGaCCCAACGU- -5' |
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| 11445 | 3' | -54.3 | NC_003085.1 | + | 29924 | 0.72 | 0.39299 |
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Target: 5'- uUCUccGCGucggGCGCcGUCCGCUGGGcUGCu -3' miRNA: 3'- -AGA--UGC----UGCGuCAGGUGACCCaACGu -5' |
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| 11445 | 3' | -54.3 | NC_003085.1 | + | 12295 | 0.88 | 0.035584 |
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Target: 5'- --gACGGCGCgguGGUCCGCUGGGUUGCGg -3' miRNA: 3'- agaUGCUGCG---UCAGGUGACCCAACGU- -5' |
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| 11445 | 3' | -54.3 | NC_003085.1 | + | 31564 | 1.09 | 0.000961 |
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Target: 5'- gUCUACGACGCAGUCCACUGGGUUGCAg -3' miRNA: 3'- -AGAUGCUGCGUCAGGUGACCCAACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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