Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11454 | 5' | -58.4 | NC_003085.1 | + | 23781 | 0.71 | 0.261971 |
Target: 5'- aGCGg-CAUGGGCgaauUCGUccgGCCGGUAGUCg -3' miRNA: 3'- -CGCagGUGCUCG----AGCG---CGGCCAUCAG- -5' |
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11454 | 5' | -58.4 | NC_003085.1 | + | 22058 | 0.72 | 0.242812 |
Target: 5'- uGCGcCCGCGGGCagguaccacgCGCGCgCGGU-GUCg -3' miRNA: 3'- -CGCaGGUGCUCGa---------GCGCG-GCCAuCAG- -5' |
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11454 | 5' | -58.4 | NC_003085.1 | + | 40702 | 0.75 | 0.147142 |
Target: 5'- aCGUCCGgGuGCUggaCGCGCCGGUGGa- -3' miRNA: 3'- cGCAGGUgCuCGA---GCGCGGCCAUCag -5' |
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11454 | 5' | -58.4 | NC_003085.1 | + | 27910 | 1.11 | 0.000314 |
Target: 5'- gGCGUCCACGAGCUCGCGCCGGUAGUCa -3' miRNA: 3'- -CGCAGGUGCUCGAGCGCGGCCAUCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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