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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11457 | 3' | -53.3 | NC_003085.1 | + | 49312 | 0.66 | 0.744845 |
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Target: 5'- -gUCCCGGGAGGCgaccuccCAGCUCCg---- -3' miRNA: 3'- aaGGGGCUCUUCGa------GUUGAGGaguuc -5' |
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| 11457 | 3' | -53.3 | NC_003085.1 | + | 33089 | 0.67 | 0.7119 |
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Target: 5'- gUgCCCGcGGAGGCUC-ACUCCUgGGu -3' miRNA: 3'- aAgGGGC-UCUUCGAGuUGAGGAgUUc -5' |
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| 11457 | 3' | -53.3 | NC_003085.1 | + | 35818 | 0.67 | 0.678194 |
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Target: 5'- cUCCCgGAGGAGCcgCAgaaGCUCCggcgcCAGGc -3' miRNA: 3'- aAGGGgCUCUUCGa-GU---UGAGGa----GUUC- -5' |
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| 11457 | 3' | -53.3 | NC_003085.1 | + | 42237 | 0.68 | 0.632648 |
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Target: 5'- gUCCCaCGGGAcGGCUCGcgcuACUCCagcggCAGGg -3' miRNA: 3'- aAGGG-GCUCU-UCGAGU----UGAGGa----GUUC- -5' |
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| 11457 | 3' | -53.3 | NC_003085.1 | + | 22393 | 0.71 | 0.477173 |
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Target: 5'- -aCCCCGAGAcggGGCgcgUCGAgUCCaUCGAGg -3' miRNA: 3'- aaGGGGCUCU---UCG---AGUUgAGG-AGUUC- -5' |
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| 11457 | 3' | -53.3 | NC_003085.1 | + | 24104 | 0.78 | 0.185903 |
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Target: 5'- aUCCuuGAGGAGUUgAGCUUCUCGGGg -3' miRNA: 3'- aAGGggCUCUUCGAgUUGAGGAGUUC- -5' |
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| 11457 | 3' | -53.3 | NC_003085.1 | + | 25353 | 1.07 | 0.001478 |
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Target: 5'- gUUCCCCGAGAAGCUCAACUCCUCAAGg -3' miRNA: 3'- -AAGGGGCUCUUCGAGUUGAGGAGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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