Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11461 | 5' | -55.5 | NC_003085.1 | + | 20547 | 0.66 | 0.657804 |
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Target: 5'- -cGGUugGCCUGCGUcuGCUGGUUgAUGCu- -3' miRNA: 3'- caCCA--CGGGCGCA--UGACCAG-UAUGca -5' |
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| 11461 | 5' | -55.5 | NC_003085.1 | + | 8607 | 0.67 | 0.612225 |
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Target: 5'- cGUGGUGacggagaaggaauCCCGCGUcCUGGcC-UACGUc -3' miRNA: 3'- -CACCAC-------------GGGCGCAuGACCaGuAUGCA- -5' |
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| 11461 | 5' | -55.5 | NC_003085.1 | + | 16174 | 0.67 | 0.569128 |
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Target: 5'- cGUGGUGgCCGC--GCUGGUUcgAgGUg -3' miRNA: 3'- -CACCACgGGCGcaUGACCAGuaUgCA- -5' |
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| 11461 | 5' | -55.5 | NC_003085.1 | + | 46889 | 0.69 | 0.483681 |
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Target: 5'- -gGGUGCCCGUGgGCUugugaaGGUCGUcaccGCGg -3' miRNA: 3'- caCCACGGGCGCaUGA------CCAGUA----UGCa -5' |
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| 11461 | 5' | -55.5 | NC_003085.1 | + | 46007 | 0.71 | 0.386473 |
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Target: 5'- aUGGUGCUCgGCGUACgUGGUCGccUGCu- -3' miRNA: 3'- cACCACGGG-CGCAUG-ACCAGU--AUGca -5' |
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| 11461 | 5' | -55.5 | NC_003085.1 | + | 24336 | 1.08 | 0.000934 |
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Target: 5'- cGUGGUGCCCGCGUACUGGUCAUACGUg -3' miRNA: 3'- -CACCACGGGCGCAUGACCAGUAUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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