miRNA: 3'- -GCGUACCCUaa--GAGGG---ACCCaCu--UCU- -5'
miRNA: 3'- gCGua-CCCUaagaGGGACCCACUUCU- -5'
miRNA: 3'- -GCGUACCCUAAGAGGGACCCACUUCU- -5'
Results 1 - 3 of 3 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11463 | 3' | -54.1 | NC_003085.1 | + | 2991 | 0.66 | 0.74661 |
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Target: 5'- gCGCGUGGGAaccgCUCCCcgcUGGGcGcacGGAc -3' miRNA: 3'- -GCGUACCCUaa--GAGGG---ACCCaCu--UCU- -5' |
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| 11463 | 3' | -54.1 | NC_003085.1 | + | 7252 | 0.69 | 0.536681 |
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Target: 5'- aGCccgGGGAcaggCUCUGGGUGAAGGa -3' miRNA: 3'- gCGua-CCCUaagaGGGACCCACUUCU- -5' |
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| 11463 | 3' | -54.1 | NC_003085.1 | + | 23975 | 1.1 | 0.000942 |
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Target: 5'- aCGCAUGGGAUUCUCCCUGGGUGAAGAa -3' miRNA: 3'- -GCGUACCCUAAGAGGGACCCACUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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