Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11465 | 5' | -53.1 | NC_003085.1 | + | 1219 | 0.67 | 0.715776 |
Target: 5'- -cCGGCGCcgcGCUGCugGAGGagUUGGa- -3' miRNA: 3'- cuGCCGUGu--UGACGugCUCC--AACUgu -5' |
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11465 | 5' | -53.1 | NC_003085.1 | + | 22123 | 0.67 | 0.715776 |
Target: 5'- aGACGGCgACGAagacgaUGUugGccGGGUUGACc -3' miRNA: 3'- -CUGCCG-UGUUg-----ACGugC--UCCAACUGu -5' |
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11465 | 5' | -53.1 | NC_003085.1 | + | 10086 | 0.66 | 0.769579 |
Target: 5'- -uUGGCGCcgGACUGUGUGAGGUgGACGc -3' miRNA: 3'- cuGCCGUG--UUGACGUGCUCCAaCUGU- -5' |
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11465 | 5' | -53.1 | NC_003085.1 | + | 38392 | 0.66 | 0.790134 |
Target: 5'- uGGCGGUGCAg--GC-CGAGGUUGGu- -3' miRNA: 3'- -CUGCCGUGUugaCGuGCUCCAACUgu -5' |
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11465 | 5' | -53.1 | NC_003085.1 | + | 23266 | 1.09 | 0.001374 |
Target: 5'- aGACGGCACAACUGCACGAGGUUGACAu -3' miRNA: 3'- -CUGCCGUGUUGACGUGCUCCAACUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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