Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11467 | 5' | -58 | NC_003085.1 | + | 33745 | 0.66 | 0.518509 |
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Target: 5'- cAAGGUGAGuCACUcgcuccaagucagGCuUGGGCGUCc -3' miRNA: 3'- cUUCCACUC-GUGGa------------CGcACCCGCAGa -5' |
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| 11467 | 5' | -58 | NC_003085.1 | + | 40882 | 0.66 | 0.4812 |
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Target: 5'- aGGAGGUGGGCAuCCUGCGcc-GCGa-- -3' miRNA: 3'- -CUUCCACUCGU-GGACGCaccCGCaga -5' |
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| 11467 | 5' | -58 | NC_003085.1 | + | 43241 | 0.67 | 0.422127 |
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Target: 5'- -----gGAGCGCCUGcCGUGGGUGa-- -3' miRNA: 3'- cuuccaCUCGUGGAC-GCACCCGCaga -5' |
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| 11467 | 5' | -58 | NC_003085.1 | + | 495 | 0.69 | 0.326057 |
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Target: 5'- cGAGGcUGAGgGCCUGCGUuuggagccGGUGUCUg -3' miRNA: 3'- cUUCC-ACUCgUGGACGCAc-------CCGCAGA- -5' |
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| 11467 | 5' | -58 | NC_003085.1 | + | 19311 | 0.73 | 0.20029 |
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Target: 5'- cGggGGUGAcGU-CCUGCGUGGGCu--- -3' miRNA: 3'- -CuuCCACU-CGuGGACGCACCCGcaga -5' |
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| 11467 | 5' | -58 | NC_003085.1 | + | 42716 | 0.73 | 0.179873 |
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Target: 5'- --cGGcG-GCACCUcagGCGUGGGCGUCg -3' miRNA: 3'- cuuCCaCuCGUGGA---CGCACCCGCAGa -5' |
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| 11467 | 5' | -58 | NC_003085.1 | + | 22324 | 1.08 | 0.000485 |
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Target: 5'- cGAAGGUGAGCACCUGCGUGGGCGUCUg -3' miRNA: 3'- -CUUCCACUCGUGGACGCACCCGCAGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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