Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11470 | 5' | -63.4 | NC_003085.1 | + | 1502 | 0.7 | 0.153315 |
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Target: 5'- -gCgGCCCGUCCgGCGCUCGG-GCa- -3' miRNA: 3'- caGgUGGGCAGGgCGUGGGCCaCGca -5' |
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| 11470 | 5' | -63.4 | NC_003085.1 | + | 10789 | 0.7 | 0.149346 |
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Target: 5'- -gCCACCUcUUCCGCGCagCGGUGCGa -3' miRNA: 3'- caGGUGGGcAGGGCGUGg-GCCACGCa -5' |
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| 11470 | 5' | -63.4 | NC_003085.1 | + | 22584 | 0.71 | 0.141685 |
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Target: 5'- aGUCCACCCa-CCgGCGCCggcaGGUGCGc -3' miRNA: 3'- -CAGGUGGGcaGGgCGUGGg---CCACGCa -5' |
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| 11470 | 5' | -63.4 | NC_003085.1 | + | 48502 | 0.71 | 0.141312 |
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Target: 5'- uGUCCugccccggcagcgGCCUGUCUgGCACCCGGacGCGg -3' miRNA: 3'- -CAGG-------------UGGGCAGGgCGUGGGCCa-CGCa -5' |
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| 11470 | 5' | -63.4 | NC_003085.1 | + | 8756 | 0.71 | 0.130868 |
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Target: 5'- gGUCCACCggcgCGUCCaGCACCCGGacgucgucccccUGCGc -3' miRNA: 3'- -CAGGUGG----GCAGGgCGUGGGCC------------ACGCa -5' |
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| 11470 | 5' | -63.4 | NC_003085.1 | + | 17803 | 0.72 | 0.11763 |
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Target: 5'- aUCCACCg--CCCGC-CCCGGUGCc- -3' miRNA: 3'- cAGGUGGgcaGGGCGuGGGCCACGca -5' |
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| 11470 | 5' | -63.4 | NC_003085.1 | + | 1483 | 0.72 | 0.114521 |
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Target: 5'- -gCCGCCCGUCaCCGuCGCcagccucggCCGGUGCGc -3' miRNA: 3'- caGGUGGGCAG-GGC-GUG---------GGCCACGCa -5' |
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| 11470 | 5' | -63.4 | NC_003085.1 | + | 43994 | 0.74 | 0.085049 |
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Target: 5'- uGUCCGCCUccaacUCCCGCACUCGGgccucgGCGg -3' miRNA: 3'- -CAGGUGGGc----AGGGCGUGGGCCa-----CGCa -5' |
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| 11470 | 5' | -63.4 | NC_003085.1 | + | 683 | 0.74 | 0.078359 |
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Target: 5'- -gCCACCUGUCCCGCGUCCGGccucggGCGc -3' miRNA: 3'- caGGUGGGCAGGGCGUGGGCCa-----CGCa -5' |
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| 11470 | 5' | -63.4 | NC_003085.1 | + | 20714 | 1.06 | 0.000272 |
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Target: 5'- aGUCCACCCGUCCCGCACCCGGUGCGUc -3' miRNA: 3'- -CAGGUGGGCAGGGCGUGGGCCACGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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