Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11471 | 5' | -54.2 | NC_003085.1 | + | 20432 | 0.66 | 0.70263 |
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Target: 5'- cUGGAcGGCGUUggagGCAUcCCCCAGCg -3' miRNA: 3'- aACUUcCUGCAGa---CGUAcGGGGUUGa -5' |
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| 11471 | 5' | -54.2 | NC_003085.1 | + | 44582 | 0.66 | 0.69141 |
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Target: 5'- -gGAGGGgACGUCagGCuaaAUGCCCCGu-- -3' miRNA: 3'- aaCUUCC-UGCAGa-CG---UACGGGGUuga -5' |
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| 11471 | 5' | -54.2 | NC_003085.1 | + | 14389 | 0.66 | 0.69141 |
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Target: 5'- aUGAGGGGCagacCcGCuaggGCCCCAACUc -3' miRNA: 3'- aACUUCCUGca--GaCGua--CGGGGUUGA- -5' |
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| 11471 | 5' | -54.2 | NC_003085.1 | + | 46449 | 0.66 | 0.680129 |
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Target: 5'- -cGucuGGGCGUCgucCGUGCgCCCAGCg -3' miRNA: 3'- aaCuu-CCUGCAGac-GUACG-GGGUUGa -5' |
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| 11471 | 5' | -54.2 | NC_003085.1 | + | 15215 | 0.66 | 0.680129 |
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Target: 5'- gUGAAGGGCGaggGCccUGCCCCggUg -3' miRNA: 3'- aACUUCCUGCagaCGu-ACGGGGuuGa -5' |
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| 11471 | 5' | -54.2 | NC_003085.1 | + | 31929 | 0.67 | 0.646038 |
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Target: 5'- -cGAAGG-CG-CUGCGaGCCCCAu-- -3' miRNA: 3'- aaCUUCCuGCaGACGUaCGGGGUuga -5' |
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| 11471 | 5' | -54.2 | NC_003085.1 | + | 9207 | 0.67 | 0.600442 |
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Target: 5'- -aGggGGGCGggacGUagGCCCCGGCg -3' miRNA: 3'- aaCuuCCUGCaga-CGuaCGGGGUUGa -5' |
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| 11471 | 5' | -54.2 | NC_003085.1 | + | 809 | 0.68 | 0.577777 |
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Target: 5'- -cGguGGACGUCcGCGUGCCgCCGc-- -3' miRNA: 3'- aaCuuCCUGCAGaCGUACGG-GGUuga -5' |
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| 11471 | 5' | -54.2 | NC_003085.1 | + | 41539 | 0.73 | 0.319523 |
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Target: 5'- gUGGAGuGACGUCUGC-UGCCgCCGcgGCg -3' miRNA: 3'- aACUUC-CUGCAGACGuACGG-GGU--UGa -5' |
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| 11471 | 5' | -54.2 | NC_003085.1 | + | 20465 | 1.06 | 0.001403 |
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Target: 5'- gUUGAAGGACGUCUGCAUGCCCCAACUg -3' miRNA: 3'- -AACUUCCUGCAGACGUACGGGGUUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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