Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11480 | 5' | -51 | NC_003085.1 | + | 25664 | 0.66 | 0.882854 |
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Target: 5'- -cGUGGAGUaCA--UCCGCAU-CGUCGu -3' miRNA: 3'- gaCACCUCA-GUaaAGGUGUGcGCAGU- -5' |
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| 11480 | 5' | -51 | NC_003085.1 | + | 36862 | 0.66 | 0.874902 |
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Target: 5'- ---cGGGG-Cg---CCACGCGCGUCAg -3' miRNA: 3'- gacaCCUCaGuaaaGGUGUGCGCAGU- -5' |
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| 11480 | 5' | -51 | NC_003085.1 | + | 47261 | 0.67 | 0.866679 |
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Target: 5'- ---aGGcGUCAccgUCCGcCACGCGUCGa -3' miRNA: 3'- gacaCCuCAGUaa-AGGU-GUGCGCAGU- -5' |
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| 11480 | 5' | -51 | NC_003085.1 | + | 24872 | 0.68 | 0.812119 |
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Target: 5'- --uUGGAGUaCGUcgUCCACGCGuCGUUg -3' miRNA: 3'- gacACCUCA-GUAa-AGGUGUGC-GCAGu -5' |
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| 11480 | 5' | -51 | NC_003085.1 | + | 13469 | 0.68 | 0.78194 |
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Target: 5'- gCUGUcgcGGAG-CGUUUCCugG-GCGUCGg -3' miRNA: 3'- -GACA---CCUCaGUAAAGGugUgCGCAGU- -5' |
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| 11480 | 5' | -51 | NC_003085.1 | + | 18370 | 0.7 | 0.683713 |
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Target: 5'- -cGUGGAGUCGcugUCCcUGCGCGUg- -3' miRNA: 3'- gaCACCUCAGUaa-AGGuGUGCGCAgu -5' |
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| 11480 | 5' | -51 | NC_003085.1 | + | 22807 | 0.7 | 0.660973 |
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Target: 5'- -cGUGGAGUCGaauccCUugGCGCGUUg -3' miRNA: 3'- gaCACCUCAGUaaa--GGugUGCGCAGu -5' |
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| 11480 | 5' | -51 | NC_003085.1 | + | 15987 | 1.1 | 0.002147 |
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Target: 5'- cCUGUGGAGUCAUUUCCACACGCGUCAa -3' miRNA: 3'- -GACACCUCAGUAAAGGUGUGCGCAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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