Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11485 | 5' | -59.6 | NC_003085.1 | + | 28192 | 0.66 | 0.461811 |
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Target: 5'- --aAGUCUGGGCGCGgcaccaccUUGuCCGUCguCCAg -3' miRNA: 3'- cucUCAGGCCCGCGU--------AAC-GGCAG--GGU- -5' |
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| 11485 | 5' | -59.6 | NC_003085.1 | + | 35589 | 0.66 | 0.451073 |
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Target: 5'- uAGAGcuugcggcuugCCGGGCGCGggUUGCCGcacauccggcacuUCCCu -3' miRNA: 3'- cUCUCa----------GGCCCGCGU--AACGGC-------------AGGGu -5' |
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| 11485 | 5' | -59.6 | NC_003085.1 | + | 38828 | 0.66 | 0.414147 |
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Target: 5'- -uGAG-CaGGGCGCAcUGCCGcgucgUCCCAc -3' miRNA: 3'- cuCUCaGgCCCGCGUaACGGC-----AGGGU- -5' |
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| 11485 | 5' | -59.6 | NC_003085.1 | + | 11792 | 0.66 | 0.414147 |
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Target: 5'- ----cUCCGGGCGUGUcgGCUGcCCCAu -3' miRNA: 3'- cucucAGGCCCGCGUAa-CGGCaGGGU- -5' |
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| 11485 | 5' | -59.6 | NC_003085.1 | + | 7329 | 0.66 | 0.414147 |
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Target: 5'- cGAGAGcaUCCGcgccGUGCAUucgcuUGCCGUCCUc -3' miRNA: 3'- -CUCUC--AGGCc---CGCGUA-----ACGGCAGGGu -5' |
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| 11485 | 5' | -59.6 | NC_003085.1 | + | 43445 | 0.67 | 0.387041 |
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Target: 5'- cGGcGUCCGGGCGUAcauCCGcgCCCAg -3' miRNA: 3'- cUCuCAGGCCCGCGUaacGGCa-GGGU- -5' |
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| 11485 | 5' | -59.6 | NC_003085.1 | + | 10565 | 0.67 | 0.387041 |
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Target: 5'- cGAGAGUCgGcGGCGCcguaguGUUGCaGUaCCCGa -3' miRNA: 3'- -CUCUCAGgC-CCGCG------UAACGgCA-GGGU- -5' |
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| 11485 | 5' | -59.6 | NC_003085.1 | + | 3482 | 0.67 | 0.378269 |
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Target: 5'- gGGGAGa--GGcGCGCAUcUGCCGcCCCAa -3' miRNA: 3'- -CUCUCaggCC-CGCGUA-ACGGCaGGGU- -5' |
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| 11485 | 5' | -59.6 | NC_003085.1 | + | 29421 | 0.69 | 0.283745 |
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Target: 5'- gGAGGG-CgCGGGCgGCGgcaaUGCCGUCCUc -3' miRNA: 3'- -CUCUCaG-GCCCG-CGUa---ACGGCAGGGu -5' |
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| 11485 | 5' | -59.6 | NC_003085.1 | + | 14254 | 1.08 | 0.000381 |
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Target: 5'- gGAGAGUCCGGGCGCAUUGCCGUCCCAg -3' miRNA: 3'- -CUCUCAGGCCCGCGUAACGGCAGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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