Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11490 | 3' | -53.6 | NC_003085.1 | + | 4118 | 0.74 | 0.32711 |
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Target: 5'- gGGCGCcgCGcugGGACUccagcgccgcucuacGGAGCGACUGCu -3' miRNA: 3'- gUCGUGa-GCa--CUUGA---------------CCUCGCUGACG- -5' |
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| 11490 | 3' | -53.6 | NC_003085.1 | + | 20666 | 0.75 | 0.27797 |
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Target: 5'- -cGCG-UCG-GAguuGCUGGAGCGGCUGCu -3' miRNA: 3'- guCGUgAGCaCU---UGACCUCGCUGACG- -5' |
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| 11490 | 3' | -53.6 | NC_003085.1 | + | 2226 | 0.75 | 0.270846 |
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Target: 5'- uGGCGCUCGUGGaaccGCUGGuGCGcaaugcCUGCc -3' miRNA: 3'- gUCGUGAGCACU----UGACCuCGCu-----GACG- -5' |
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| 11490 | 3' | -53.6 | NC_003085.1 | + | 32120 | 0.77 | 0.19649 |
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Target: 5'- aCGGCACUCGgugGuGCgacgGGGGCGGCUGg -3' miRNA: 3'- -GUCGUGAGCa--CuUGa---CCUCGCUGACg -5' |
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| 11490 | 3' | -53.6 | NC_003085.1 | + | 12360 | 1.13 | 0.000693 |
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Target: 5'- cCAGCACUCGUGAACUGGAGCGACUGCg -3' miRNA: 3'- -GUCGUGAGCACUUGACCUCGCUGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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