Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11493 | 5' | -50.3 | NC_003085.1 | + | 3116 | 0.66 | 0.919852 |
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Target: 5'- gAGGCGa--UGCUCGaccUUGAGAGgcGCg -3' miRNA: 3'- gUUCGCaacACGAGC---AGCUCUCaaCG- -5' |
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| 11493 | 5' | -50.3 | NC_003085.1 | + | 7494 | 0.66 | 0.906623 |
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Target: 5'- aCGGGCGUccg---CGUCGAGcGGUUGCa -3' miRNA: 3'- -GUUCGCAacacgaGCAGCUC-UCAACG- -5' |
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| 11493 | 5' | -50.3 | NC_003085.1 | + | 43428 | 0.66 | 0.906623 |
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Target: 5'- aGAGCGUc--GCgUCGUCGuGAGgggUGCc -3' miRNA: 3'- gUUCGCAacaCG-AGCAGCuCUCa--ACG- -5' |
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| 11493 | 5' | -50.3 | NC_003085.1 | + | 11221 | 0.67 | 0.873407 |
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Target: 5'- gUAGGUGgccaccgccgacGUGCUgcCGUCGAGAGU-GCa -3' miRNA: 3'- -GUUCGCaa----------CACGA--GCAGCUCUCAaCG- -5' |
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| 11493 | 5' | -50.3 | NC_003085.1 | + | 14885 | 0.69 | 0.782764 |
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Target: 5'- aCGAGCag-GUGUUCGcCGccauugaGGAGUUGCg -3' miRNA: 3'- -GUUCGcaaCACGAGCaGC-------UCUCAACG- -5' |
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| 11493 | 5' | -50.3 | NC_003085.1 | + | 34355 | 0.72 | 0.639632 |
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Target: 5'- -cGGCGcuccauccGCUCGUCGAGGGUgagGCg -3' miRNA: 3'- guUCGCaaca----CGAGCAGCUCUCAa--CG- -5' |
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| 11493 | 5' | -50.3 | NC_003085.1 | + | 10878 | 1.14 | 0.001362 |
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Target: 5'- aCAAGCGUUGUGCUCGUCGAGAGUUGCa -3' miRNA: 3'- -GUUCGCAACACGAGCAGCUCUCAACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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