Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11497 | 3' | -56.1 | NC_003085.1 | + | 47375 | 0.66 | 0.627821 |
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Target: 5'- -cGaGGUGcgGCGGcUGcGCUCgUCGCg -3' miRNA: 3'- guC-CCACuaCGCCuACuCGAGgAGUG- -5' |
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| 11497 | 3' | -56.1 | NC_003085.1 | + | 42674 | 0.66 | 0.616669 |
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Target: 5'- -cGGGUGAgacucagguaGCGGAaGGGCU-CUCGCc -3' miRNA: 3'- guCCCACUa---------CGCCUaCUCGAgGAGUG- -5' |
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| 11497 | 3' | -56.1 | NC_003085.1 | + | 31667 | 0.66 | 0.605531 |
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Target: 5'- cCGGGGcgggcggugGAUGUGGGgugucaUGAGCgggCCUCAg -3' miRNA: 3'- -GUCCCa--------CUACGCCU------ACUCGa--GGAGUg -5' |
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| 11497 | 3' | -56.1 | NC_003085.1 | + | 16610 | 0.67 | 0.550374 |
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Target: 5'- gAGGG-GAUGUGGcAUGAGCaCgCUCAa -3' miRNA: 3'- gUCCCaCUACGCC-UACUCGaG-GAGUg -5' |
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| 11497 | 3' | -56.1 | NC_003085.1 | + | 7041 | 0.67 | 0.53951 |
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Target: 5'- gCGGGGUGG-GC-GAUGAGC-CCgCGCg -3' miRNA: 3'- -GUCCCACUaCGcCUACUCGaGGaGUG- -5' |
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| 11497 | 3' | -56.1 | NC_003085.1 | + | 32556 | 0.68 | 0.486426 |
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Target: 5'- gCGGGGgcuggGGUGCGaGGUG-GCUCaUCGCu -3' miRNA: 3'- -GUCCCa----CUACGC-CUACuCGAGgAGUG- -5' |
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| 11497 | 3' | -56.1 | NC_003085.1 | + | 34190 | 0.7 | 0.370663 |
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Target: 5'- -cGGGcGcgGCGGGUGAGCUCggcCGCg -3' miRNA: 3'- guCCCaCuaCGCCUACUCGAGga-GUG- -5' |
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| 11497 | 3' | -56.1 | NC_003085.1 | + | 37162 | 0.72 | 0.297055 |
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Target: 5'- uGGcGGUGAcGgGGAcGAGCUCCUuCACg -3' miRNA: 3'- gUC-CCACUaCgCCUaCUCGAGGA-GUG- -5' |
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| 11497 | 3' | -56.1 | NC_003085.1 | + | 9700 | 1.09 | 0.000569 |
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Target: 5'- uCAGGGUGAUGCGGAUGAGCUCCUCACg -3' miRNA: 3'- -GUCCCACUACGCCUACUCGAGGAGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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