Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 115 | 5' | -52.6 | AC_000006.1 | + | 23045 | 0.66 | 0.729381 |
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Target: 5'- gGUCGGCCAGggcgucccgGAG-GAUgaGGGACUg -3' miRNA: 3'- -CAGUCGGUCaaa------CUCaCUG--UCCUGGg -5' |
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| 115 | 5' | -52.6 | AC_000006.1 | + | 32689 | 0.66 | 0.706805 |
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Target: 5'- -gCAGUguGUUcaUGAGgGGCAGGcACCUg -3' miRNA: 3'- caGUCGguCAA--ACUCaCUGUCC-UGGG- -5' |
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| 115 | 5' | -52.6 | AC_000006.1 | + | 10471 | 0.67 | 0.649167 |
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Target: 5'- aUC-GCCAGggUUGAGUcgcGGCAGaACCCg -3' miRNA: 3'- cAGuCGGUCa-AACUCA---CUGUCcUGGG- -5' |
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| 115 | 5' | -52.6 | AC_000006.1 | + | 9983 | 0.7 | 0.45762 |
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Target: 5'- -gCGGCCAGcgcUGGGUGGCcGGcgcGCCCg -3' miRNA: 3'- caGUCGGUCaa-ACUCACUGuCC---UGGG- -5' |
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| 115 | 5' | -52.6 | AC_000006.1 | + | 9123 | 0.71 | 0.406843 |
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Target: 5'- --gGGgCGGUgguGGUGGCGGGGCCCg -3' miRNA: 3'- cagUCgGUCAaacUCACUGUCCUGGG- -5' |
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| 115 | 5' | -52.6 | AC_000006.1 | + | 16505 | 0.76 | 0.22301 |
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Target: 5'- aUCAGCCGGU-----UGGCGGGGCCCc -3' miRNA: 3'- cAGUCGGUCAaacucACUGUCCUGGG- -5' |
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| 115 | 5' | -52.6 | AC_000006.1 | + | 31095 | 1.11 | 0.000703 |
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Target: 5'- gGUCAGCCAGUUUGAGUGACAGGACCCc -3' miRNA: 3'- -CAGUCGGUCAAACUCACUGUCCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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