Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11502 | 3' | -58.9 | NC_003085.1 | + | 46454 | 0.7 | 0.278126 |
Target: 5'- -gGGCGUCG--UCCgUG-CGCCCAGCg -3' miRNA: 3'- caCCGUAGUgaGGGgACaGCGGGUCG- -5' |
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11502 | 3' | -58.9 | NC_003085.1 | + | 46611 | 0.74 | 0.136162 |
Target: 5'- -cGGCAUgGCUCagCCCUccUCGCCCGGCu -3' miRNA: 3'- caCCGUAgUGAG--GGGAc-AGCGGGUCG- -5' |
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11502 | 3' | -58.9 | NC_003085.1 | + | 46824 | 0.66 | 0.455234 |
Target: 5'- -cGGCAUCACcucgCCgCCgaGUUGCgacgCCAGCg -3' miRNA: 3'- caCCGUAGUGa---GG-GGa-CAGCG----GGUCG- -5' |
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11502 | 3' | -58.9 | NC_003085.1 | + | 49113 | 0.66 | 0.455234 |
Target: 5'- -cGGCGcUCA-UCCCUUGcCGCCCuucuuGGCg -3' miRNA: 3'- caCCGU-AGUgAGGGGACaGCGGG-----UCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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