Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11502 | 5' | -60.7 | NC_003085.1 | + | 37153 | 0.66 | 0.363063 |
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Target: 5'- cGGGCacccUGGCgGUGACGGGGacGAGcUCCu- -3' miRNA: 3'- -UCCG----ACCGaCGCUGCCCU--CUC-AGGua -5' |
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| 11502 | 5' | -60.7 | NC_003085.1 | + | 1322 | 0.66 | 0.354643 |
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Target: 5'- aAGGCUGGCacUGCGAUGGcGcuGGAGUaCGg -3' miRNA: 3'- -UCCGACCG--ACGCUGCC-C--UCUCAgGUa -5' |
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| 11502 | 5' | -60.7 | NC_003085.1 | + | 27022 | 0.66 | 0.354643 |
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Target: 5'- cGGGCUGGCgcuccuCGACGGacuGGuAGUCCGg -3' miRNA: 3'- -UCCGACCGac----GCUGCCc--UC-UCAGGUa -5' |
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| 11502 | 5' | -60.7 | NC_003085.1 | + | 10361 | 0.66 | 0.346363 |
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Target: 5'- cGGCcaGGaCUGCGGCGGGAGcGUUg-- -3' miRNA: 3'- uCCGa-CC-GACGCUGCCCUCuCAGgua -5' |
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| 11502 | 5' | -60.7 | NC_003085.1 | + | 8821 | 0.67 | 0.32236 |
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Target: 5'- gAGGCugccgagacaUGGCUGCGcgaGCaGGAGGGgcggCCAUg -3' miRNA: 3'- -UCCG----------ACCGACGC---UGcCCUCUCa---GGUA- -5' |
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| 11502 | 5' | -60.7 | NC_003085.1 | + | 19027 | 0.67 | 0.32236 |
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Target: 5'- cGGCaaguccucUGGCUGCGuCGuGGAGAuUCCGc -3' miRNA: 3'- uCCG--------ACCGACGCuGC-CCUCUcAGGUa -5' |
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| 11502 | 5' | -60.7 | NC_003085.1 | + | 42781 | 0.69 | 0.232823 |
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Target: 5'- cGGCuUGaGCcucgGUGACGGGAGGGUCgAa -3' miRNA: 3'- uCCG-AC-CGa---CGCUGCCCUCUCAGgUa -5' |
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| 11502 | 5' | -60.7 | NC_003085.1 | + | 14374 | 0.7 | 0.209822 |
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Target: 5'- cGGCUGGCUGCG-CGGauGAGGGgcagacCCGc -3' miRNA: 3'- uCCGACCGACGCuGCC--CUCUCa-----GGUa -5' |
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| 11502 | 5' | -60.7 | NC_003085.1 | + | 47979 | 0.71 | 0.18385 |
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Target: 5'- gAGGCUGGCgacgGUGACGGGcggcgacuuGGGGUgUCAUg -3' miRNA: 3'- -UCCGACCGa---CGCUGCCC---------UCUCA-GGUA- -5' |
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| 11502 | 5' | -60.7 | NC_003085.1 | + | 7290 | 1.06 | 0.000365 |
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Target: 5'- gAGGCUGGCUGCGACGGGAGAGUCCAUg -3' miRNA: 3'- -UCCGACCGACGCUGCCCUCUCAGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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