miRNA: 3'- gUUCGCGCGGgaCuu---CCacuUCUGCG- -5'
miRNA: 3'- gUUCGC-GCG-GGacuuCCAC----------UUCUGCG- -5'
miRNA: 3'- guUCGCGCGGGacuuCCACUUcUGCG- -5'
miRNA: 3'- guuCGCGCGGga---CuuCCACuuCUGCG- -5'
miRNA: 3'- gUUCGCGCGGGaCUUCcACU--------------UCUGCG- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11509 | 3' | -54.7 | NC_003085.1 | + | 11888 | 0.66 | 0.723454 |
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Target: 5'- uGAGCGCGCCgaGuucauGGcc-AGGCGCu -3' miRNA: 3'- gUUCGCGCGGgaCuu---CCacuUCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 15123 | 0.66 | 0.723454 |
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Target: 5'- gGAGCGcCGCgCCgcguGGUGcgacgagcacGAGACGCa -3' miRNA: 3'- gUUCGC-GCG-GGacuuCCAC----------UUCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 27369 | 0.66 | 0.723454 |
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Target: 5'- -cAGCGCGUCUggcuGGUGGA-ACGCg -3' miRNA: 3'- guUCGCGCGGGacuuCCACUUcUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 28505 | 0.66 | 0.723454 |
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Target: 5'- --cGCGCGCCauucaGgcGGUG--GACGCg -3' miRNA: 3'- guuCGCGCGGga---CuuCCACuuCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 620 | 0.65 | 0.730987 |
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Target: 5'- uGGGCGCGCUC-GAGGcUGGcgcggaccucgucgAGGCGCu -3' miRNA: 3'- gUUCGCGCGGGaCUUCcACU--------------UCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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