miRNA: 3'- guUCGCGCGGGa-CUUCCACU---------UCUGCG- -5'
miRNA: 3'- guuCgCGCGGGACUUCCaCuuCUGCG- -5'
miRNA: 3'- -GUUCGCGCGgGa-CuuCCACUuCUGCG- -5'
miRNA: 3'- -GUUCGCGCGGGACUUccacuuCUGCG- -5'
miRNA: 3'- -GUUCGCGCGGGACUUCCACUUCUGCG- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11509 | 3' | -54.7 | NC_003085.1 | + | 9046 | 0.76 | 0.220693 |
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Target: 5'- -cAGCGCGCCUccGAAGGUGGugaggaguuGGAUGCc -3' miRNA: 3'- guUCGCGCGGGa-CUUCCACU---------UCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 33203 | 0.76 | 0.214858 |
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Target: 5'- --cGgGCGUCCUGGAGGcGgcGGCGCa -3' miRNA: 3'- guuCgCGCGGGACUUCCaCuuCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 26709 | 0.77 | 0.177632 |
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Target: 5'- uCGAGCGCGCgCagGgcGGUGAcGGCGCg -3' miRNA: 3'- -GUUCGCGCGgGa-CuuCCACUuCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 39720 | 0.77 | 0.172807 |
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Target: 5'- cCAAGCGCGCCCUGGAcuccauGACGCc -3' miRNA: 3'- -GUUCGCGCGGGACUUccacuuCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 1435 | 1.11 | 0.000732 |
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Target: 5'- cCAAGCGCGCCCUGAAGGUGAAGACGCc -3' miRNA: 3'- -GUUCGCGCGGGACUUCCACUUCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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