Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11509 | 3' | -54.7 | NC_003085.1 | + | 40560 | 0.67 | 0.668555 |
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Target: 5'- gAGGCGCaagGCCUUGGuggcGGUGcgcaugccAGACGCg -3' miRNA: 3'- gUUCGCG---CGGGACUu---CCACu-------UCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 5319 | 0.67 | 0.657423 |
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Target: 5'- gCGAGCGCGUggccaCgCUGGAGGcGAAGucaGCa -3' miRNA: 3'- -GUUCGCGCG-----G-GACUUCCaCUUCug-CG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 12656 | 0.67 | 0.657423 |
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Target: 5'- --uGuCGCGCUCgccGGaGUGGAGACGCu -3' miRNA: 3'- guuC-GCGCGGGacuUC-CACUUCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 27775 | 0.67 | 0.646268 |
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Target: 5'- gAAGCGggccaccuCGUCCUGGAGGcgcUGAagccGGAUGCu -3' miRNA: 3'- gUUCGC--------GCGGGACUUCC---ACU----UCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 7208 | 0.66 | 0.723454 |
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Target: 5'- ---cCGCGCCCUGccGcUGGAGuaGCGCg -3' miRNA: 3'- guucGCGCGGGACuuCcACUUC--UGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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