miRNA: 3'- guucGCGCGGG--ACuuCCACUUCugCG- -5'
miRNA: 3'- gUUCGCGCGgGaCU---UCCa---CUUCUGCG- -5'
miRNA: 3'- -GUUCGCGCGGGa-----CUUCca---CuUCUGCG- -5'
miRNA: 3'- gUUCGCGCgGGACUUC-C-AC-----------UUCUGCG- -5'
miRNA: 3'- guUCGCgCGGGACUUCCacuucUGCG- -5'
Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11509 | 3' | -54.7 | NC_003085.1 | + | 7605 | 0.67 | 0.646268 |
|
Target: 5'- ---aCGCGUCCgaUGucGGUGAAGugGUu -3' miRNA: 3'- guucGCGCGGG--ACuuCCACUUCugCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 13572 | 0.67 | 0.646268 |
|
Target: 5'- aAGGCGCGCgUgGAcguGGGcaccGGAGGCGCg -3' miRNA: 3'- gUUCGCGCGgGaCU---UCCa---CUUCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 31307 | 0.67 | 0.646268 |
|
Target: 5'- gCAGGCGCGCCCgacaccGAAGccucaGcAGACGUc -3' miRNA: 3'- -GUUCGCGCGGGa-----CUUCca---CuUCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 3104 | 0.67 | 0.657423 |
|
Target: 5'- aAGGCGCGgCUUGAGGcGaUGcucgaccuugaGAGGCGCg -3' miRNA: 3'- gUUCGCGCgGGACUUC-C-AC-----------UUCUGCG- -5' |
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| 11509 | 3' | -54.7 | NC_003085.1 | + | 19689 | 0.66 | 0.723454 |
|
Target: 5'- -uGGCG-GCCCUGGAGGc----GCGCg -3' miRNA: 3'- guUCGCgCGGGACUUCCacuucUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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