Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11515 | 5' | -62.4 | NC_003085.1 | + | 42532 | 0.66 | 0.361122 |
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Target: 5'- -gCGUgGCCuccCGCUucGCCUCGGCCa -3' miRNA: 3'- ggGCAgUGGca-GCGGu-CGGAGCCGGc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 14114 | 0.66 | 0.386023 |
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Target: 5'- -aCGgacaCGCCGUCGCgCAGUagCUCGGCa- -3' miRNA: 3'- ggGCa---GUGGCAGCG-GUCG--GAGCCGgc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 46832 | 0.66 | 0.386023 |
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Target: 5'- cCUCGcCGCCGaguugcgaCGCCagcgucugGGCCUUGGCCu -3' miRNA: 3'- -GGGCaGUGGCa-------GCGG--------UCGGAGCCGGc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 36766 | 0.66 | 0.386023 |
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Target: 5'- gCCGgccUCGCCGUCGgCGuacuCCUCGGCg- -3' miRNA: 3'- gGGC---AGUGGCAGCgGUc---GGAGCCGgc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 33931 | 0.66 | 0.386023 |
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Target: 5'- --aGUCGCCGUgcaguugacuaCGUgGGCCggUGGCCGg -3' miRNA: 3'- gggCAGUGGCA-----------GCGgUCGGa-GCCGGC- -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 9764 | 0.66 | 0.386872 |
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Target: 5'- cUCCaGUCugCaGggaCGCCAGCCggugcucgacguuggCGGCCGu -3' miRNA: 3'- -GGG-CAGugG-Ca--GCGGUCGGa--------------GCCGGC- -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 32995 | 0.66 | 0.394571 |
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Target: 5'- gCCgGUCGCCGUUGCCGcauucgcgacGUCgaaggcggaCGGCCc -3' miRNA: 3'- -GGgCAGUGGCAGCGGU----------CGGa--------GCCGGc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 2296 | 0.66 | 0.394571 |
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Target: 5'- cUCCGgcagCACgG-CGCUGGCCUgccucgccacCGGCCGc -3' miRNA: 3'- -GGGCa---GUGgCaGCGGUCGGA----------GCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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