Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11515 | 5' | -62.4 | NC_003085.1 | + | 42967 | 0.71 | 0.174258 |
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Target: 5'- uCCCGcaUCAuCCGUgaCGCUGGCCacuucaUCGGCCGa -3' miRNA: 3'- -GGGC--AGU-GGCA--GCGGUCGG------AGCCGGC- -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 45028 | 0.75 | 0.092437 |
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Target: 5'- gCUCGUCagggaugcucuGCCaGUCGCCAGCCUCcuccGCCGg -3' miRNA: 3'- -GGGCAG-----------UGG-CAGCGGUCGGAGc---CGGC- -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 45662 | 0.74 | 0.102942 |
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Target: 5'- gCCCaG-CACCGUCGCC-GCUUCGGCg- -3' miRNA: 3'- -GGG-CaGUGGCAGCGGuCGGAGCCGgc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 46684 | 0.66 | 0.361122 |
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Target: 5'- gCCUGgagCGCCGcCGCU-GCCUgcuucCGGCCa -3' miRNA: 3'- -GGGCa--GUGGCaGCGGuCGGA-----GCCGGc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 46832 | 0.66 | 0.386023 |
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Target: 5'- cCUCGcCGCCGaguugcgaCGCCagcgucugGGCCUUGGCCu -3' miRNA: 3'- -GGGCaGUGGCa-------GCGG--------UCGGAGCCGGc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 48100 | 0.69 | 0.2539 |
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Target: 5'- gCCCGUCGCUGUCGUa---CUUGGCgGc -3' miRNA: 3'- -GGGCAGUGGCAGCGgucgGAGCCGgC- -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 48902 | 0.73 | 0.124071 |
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Target: 5'- gCCCGcCAgCCG-CGCC-GCCUCGcGCCGu -3' miRNA: 3'- -GGGCaGU-GGCaGCGGuCGGAGC-CGGC- -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 49025 | 0.67 | 0.314761 |
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Target: 5'- gUCCuUCACCG-CGaCCAGCgacucUUCGGCCa -3' miRNA: 3'- -GGGcAGUGGCaGC-GGUCG-----GAGCCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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