Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11515 | 5' | -62.4 | NC_003085.1 | + | 34815 | 0.67 | 0.329699 |
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Target: 5'- aCCCGUCACCuccgCGCgAuGCCaUCGGUa- -3' miRNA: 3'- -GGGCAGUGGca--GCGgU-CGG-AGCCGgc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 14786 | 0.67 | 0.307485 |
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Target: 5'- -aCGUCcCUGUCggGCUGGCCuUCGGCCu -3' miRNA: 3'- ggGCAGuGGCAG--CGGUCGG-AGCCGGc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 12097 | 0.67 | 0.300339 |
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Target: 5'- gUCGcgcagCACCGgcaggugCGCCAGCgUCaGGCCGc -3' miRNA: 3'- gGGCa----GUGGCa------GCGGUCGgAG-CCGGC- -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 18486 | 0.68 | 0.293322 |
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Target: 5'- cCCUGUCgGCCaGUCGCUucGCCUCGGg-- -3' miRNA: 3'- -GGGCAG-UGG-CAGCGGu-CGGAGCCggc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 1404 | 0.68 | 0.293322 |
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Target: 5'- gUCCGUacuccagcgcCAUCGcagUGCCAGCCUucgCGGCCa -3' miRNA: 3'- -GGGCA----------GUGGCa--GCGGUCGGA---GCCGGc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 42269 | 0.68 | 0.273041 |
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Target: 5'- cCCUGUCGCCcagCGCCucauGCCgaGGCUGg -3' miRNA: 3'- -GGGCAGUGGca-GCGGu---CGGagCCGGC- -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 40902 | 0.69 | 0.2539 |
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Target: 5'- gCCCGgcCGCCGUCagguacagGCCGGCCgggacgcgcgUGGCCu -3' miRNA: 3'- -GGGCa-GUGGCAG--------CGGUCGGa---------GCCGGc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 32995 | 0.66 | 0.394571 |
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Target: 5'- gCCgGUCGCCGUUGCCGcauucgcgacGUCgaaggcggaCGGCCc -3' miRNA: 3'- -GGgCAGUGGCAGCGGU----------CGGa--------GCCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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