Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11515 | 5' | -62.4 | NC_003085.1 | + | 25863 | 0.66 | 0.353074 |
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Target: 5'- aUCGUCACCGUCGagCAacucGCCgcCGuGCCGg -3' miRNA: 3'- gGGCAGUGGCAGCg-GU----CGGa-GC-CGGC- -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 4432 | 0.66 | 0.345154 |
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Target: 5'- gCCCGacggugcuccUCACCGUCGCCG---UCGGCg- -3' miRNA: 3'- -GGGC----------AGUGGCAGCGGUcggAGCCGgc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 42532 | 0.66 | 0.361122 |
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Target: 5'- -gCGUgGCCuccCGCUucGCCUCGGCCa -3' miRNA: 3'- ggGCAgUGGca-GCGGu-CGGAGCCGGc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 49025 | 0.67 | 0.314761 |
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Target: 5'- gUCCuUCACCG-CGaCCAGCgacucUUCGGCCa -3' miRNA: 3'- -GGGcAGUGGCaGC-GGUCG-----GAGCCGGc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 16493 | 0.67 | 0.314027 |
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Target: 5'- uCCCGUCGCUGacggCGCCgaggaagGGUgUCGGCa- -3' miRNA: 3'- -GGGCAGUGGCa---GCGG-------UCGgAGCCGgc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 41651 | 0.67 | 0.307485 |
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Target: 5'- gCCGcUCACCuggUGCUGuGCCUUGGCCa -3' miRNA: 3'- gGGC-AGUGGca-GCGGU-CGGAGCCGGc -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 40798 | 0.67 | 0.299632 |
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Target: 5'- cCCCGgCGCCcaaggccacgcgcGUCccgGCCGGCCUguaccugacggCGGCCGg -3' miRNA: 3'- -GGGCaGUGG-------------CAG---CGGUCGGA-----------GCCGGC- -5' |
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| 11515 | 5' | -62.4 | NC_003085.1 | + | 1486 | 1.1 | 0.00019 |
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Target: 5'- gCCCGUCACCGUCGCCAGCCUCGGCCGg -3' miRNA: 3'- -GGGCAGUGGCAGCGGUCGGAGCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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