Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11516 | 5' | -56 | NC_003085.1 | + | 29835 | 0.66 | 0.643286 |
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Target: 5'- -gGCCaGUGcGCAcGAGGuGGgGCGCGa -3' miRNA: 3'- caCGG-CACaCGUaCUCCuCUgCGUGC- -5' |
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| 11516 | 5' | -56 | NC_003085.1 | + | 46070 | 0.66 | 0.632195 |
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Target: 5'- -gGCCGUcGU-CAcccugUGGGGAGAgGCGCGc -3' miRNA: 3'- caCGGCA-CAcGU-----ACUCCUCUgCGUGC- -5' |
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| 11516 | 5' | -56 | NC_003085.1 | + | 16801 | 0.66 | 0.621104 |
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Target: 5'- uUGuuGUGUGCG-GAGGcccgcAGACGguCGu -3' miRNA: 3'- cACggCACACGUaCUCC-----UCUGCguGC- -5' |
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| 11516 | 5' | -56 | NC_003085.1 | + | 15132 | 0.67 | 0.576933 |
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Target: 5'- -cGCCGcgugGUGCGacGAGcacGAGACGCACu -3' miRNA: 3'- caCGGCa---CACGUa-CUC---CUCUGCGUGc -5' |
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| 11516 | 5' | -56 | NC_003085.1 | + | 39445 | 0.67 | 0.576932 |
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Target: 5'- -cGCCGgacUGUGUGAGGuGGACGC-CGu -3' miRNA: 3'- caCGGCac-ACGUACUCC-UCUGCGuGC- -5' |
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| 11516 | 5' | -56 | NC_003085.1 | + | 3646 | 0.67 | 0.565983 |
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Target: 5'- aUGCUcgGUG-GCA-GAcGGAGugGCACGa -3' miRNA: 3'- cACGG--CACaCGUaCU-CCUCugCGUGC- -5' |
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| 11516 | 5' | -56 | NC_003085.1 | + | 17629 | 0.67 | 0.541019 |
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Target: 5'- cGUGCCGgagcaccugcgcagGCGUGAgccacgggagGGAGGCGCugGc -3' miRNA: 3'- -CACGGCaca-----------CGUACU----------CCUCUGCGugC- -5' |
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| 11516 | 5' | -56 | NC_003085.1 | + | 1896 | 0.68 | 0.491274 |
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Target: 5'- -aGCCG-GUGCGcuucaacAGGGGGCGCACc -3' miRNA: 3'- caCGGCaCACGUac-----UCCUCUGCGUGc -5' |
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| 11516 | 5' | -56 | NC_003085.1 | + | 7516 | 0.69 | 0.470749 |
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Target: 5'- --aCCGgg-GCAUGuggauGGAGACGCGCGu -3' miRNA: 3'- cacGGCacaCGUACu----CCUCUGCGUGC- -5' |
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| 11516 | 5' | -56 | NC_003085.1 | + | 1598 | 1.1 | 0.000619 |
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Target: 5'- cGUGCCGUGUGCAUGAGGAGACGCACGg -3' miRNA: 3'- -CACGGCACACGUACUCCUCUGCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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