Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11517 | 3' | -57.7 | NC_003085.1 | + | 16579 | 0.66 | 0.534374 |
Target: 5'- ----aGUGCCGaCACC-CUuccucgGCGCCGUCa -3' miRNA: 3'- ugagaCACGGC-GUGGcGA------UGCGGUAG- -5' |
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11517 | 3' | -57.7 | NC_003085.1 | + | 22846 | 0.66 | 0.545097 |
Target: 5'- uGCcgCUGccGUCGcCACCGCggcggGCGUCGUCg -3' miRNA: 3'- -UGa-GACa-CGGC-GUGGCGa----UGCGGUAG- -5' |
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11517 | 3' | -57.7 | NC_003085.1 | + | 5635 | 0.66 | 0.545097 |
Target: 5'- ----cGUGgCGCACCGUcgcgGCGCCAg- -3' miRNA: 3'- ugagaCACgGCGUGGCGa---UGCGGUag -5' |
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11517 | 3' | -57.7 | NC_003085.1 | + | 23201 | 0.71 | 0.253559 |
Target: 5'- aGCUCUGcGCCGUcuuGCggUGCUGCGCCGccUCg -3' miRNA: 3'- -UGAGACaCGGCG---UG--GCGAUGCGGU--AG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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