Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11518 | 5' | -53.3 | NC_003085.1 | + | 37697 | 0.67 | 0.76818 |
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Target: 5'- aGGCGguaccACGUCaaugcGCGUCGACUGCuacgacggaaccccAGCg -3' miRNA: 3'- -CCGC-----UGCAGa----CGCAGCUGAUGu-------------UCGa -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 39027 | 0.68 | 0.710774 |
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Target: 5'- aGGCGACGgcaUGCGUgGGC---GAGCg -3' miRNA: 3'- -CCGCUGCag-ACGCAgCUGaugUUCGa -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 41050 | 0.66 | 0.784465 |
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Target: 5'- cGGCuuGGCGggCUGCuacgCGACUGgGGGCUg -3' miRNA: 3'- -CCG--CUGCa-GACGca--GCUGAUgUUCGA- -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 48183 | 0.67 | 0.764049 |
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Target: 5'- uGGCGACGUUgccGCcaaGUaCGACagcgACGGGCUg -3' miRNA: 3'- -CCGCUGCAGa--CG---CA-GCUGa---UGUUCGA- -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 48391 | 0.66 | 0.774333 |
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Target: 5'- cGGCGAgcagcgccgcauUGUCUGCcUCGGCgGCGcuGGCUu -3' miRNA: 3'- -CCGCU------------GCAGACGcAGCUGaUGU--UCGA- -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 48958 | 0.75 | 0.307774 |
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Target: 5'- uGGCGGgccaaugcucCGUgUGCGgCGACUGCAAGCc -3' miRNA: 3'- -CCGCU----------GCAgACGCaGCUGAUGUUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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