Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11518 | 5' | -53.3 | NC_003085.1 | + | 48183 | 0.67 | 0.764049 |
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Target: 5'- uGGCGACGUUgccGCcaaGUaCGACagcgACGGGCUg -3' miRNA: 3'- -CCGCUGCAGa--CG---CA-GCUGa---UGUUCGA- -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 3407 | 0.66 | 0.784465 |
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Target: 5'- aGCGuCGUCUGUG-CGGCgACcAGCg -3' miRNA: 3'- cCGCuGCAGACGCaGCUGaUGuUCGa -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 33940 | 0.66 | 0.784465 |
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Target: 5'- uGGCGAacgaGUCgccgUGCaGUUGACUACGugGGCc -3' miRNA: 3'- -CCGCUg---CAG----ACG-CAGCUGAUGU--UCGa -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 26571 | 0.66 | 0.798373 |
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Target: 5'- aGGCGAUGUCggccacagacuccgGCGUgGGgUGCA-GCa -3' miRNA: 3'- -CCGCUGCAGa-------------CGCAgCUgAUGUuCGa -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 11633 | 0.66 | 0.804228 |
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Target: 5'- gGGCagGACGg-UGCGUUGGC-GCAGGCc -3' miRNA: 3'- -CCG--CUGCagACGCAGCUGaUGUUCGa -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 30221 | 0.78 | 0.197019 |
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Target: 5'- gGGUGACGUcCUGCGUgGGCUGCugGGGCc -3' miRNA: 3'- -CCGCUGCA-GACGCAgCUGAUG--UUCGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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