Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11518 | 5' | -53.3 | NC_003085.1 | + | 37323 | 0.7 | 0.566412 |
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Target: 5'- cGUGugGUCUGCGaCGcCUGCcuuGAGCg -3' miRNA: 3'- cCGCugCAGACGCaGCuGAUG---UUCGa -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 1551 | 0.7 | 0.555429 |
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Target: 5'- uGGCGACGgugacggGCGgCGACUugGGGUg -3' miRNA: 3'- -CCGCUGCaga----CGCaGCUGAugUUCGa -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 48958 | 0.75 | 0.307774 |
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Target: 5'- uGGCGGgccaaugcucCGUgUGCGgCGACUGCAAGCc -3' miRNA: 3'- -CCGCU----------GCAgACGCaGCUGAUGUUCGa -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 6324 | 0.78 | 0.213663 |
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Target: 5'- cGGCGGCGUCguguagGCGUCGACcAUgucgGAGCg -3' miRNA: 3'- -CCGCUGCAGa-----CGCAGCUGaUG----UUCGa -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 30221 | 0.78 | 0.197019 |
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Target: 5'- gGGUGACGUcCUGCGUgGGCUGCugGGGCc -3' miRNA: 3'- -CCGCUGCA-GACGCAgCUGAUG--UUCGa -5' |
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| 11518 | 5' | -53.3 | NC_003085.1 | + | 3482 | 1.1 | 0.001289 |
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Target: 5'- cGGCGACGUCUGCGUCGACUACAAGCUc -3' miRNA: 3'- -CCGCUGCAGACGCAGCUGAUGUUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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