Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11520 | 5' | -55.8 | NC_003085.1 | + | 7385 | 0.66 | 0.658182 |
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Target: 5'- gGCcGaCGGCGCCcgcgUGAACUGGcacGAGUCc -3' miRNA: 3'- aCGaC-GCUGCGGa---GCUUGACC---UUCGG- -5' |
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| 11520 | 5' | -55.8 | NC_003085.1 | + | 24626 | 0.66 | 0.658182 |
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Target: 5'- gUGCUGCaGCGCUUCaccucGGCggaGGAGGCg -3' miRNA: 3'- -ACGACGcUGCGGAGc----UUGa--CCUUCGg -5' |
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| 11520 | 5' | -55.8 | NC_003085.1 | + | 10491 | 0.66 | 0.658182 |
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Target: 5'- cUGCUGCGcgGCGCaCUgGAGaauccgcGGAAGCg -3' miRNA: 3'- -ACGACGC--UGCG-GAgCUUga-----CCUUCGg -5' |
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| 11520 | 5' | -55.8 | NC_003085.1 | + | 41408 | 0.66 | 0.658182 |
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Target: 5'- gUGUUGuCGGaGCCUCGGGC-GGcaacAGCCa -3' miRNA: 3'- -ACGAC-GCUgCGGAGCUUGaCCu---UCGG- -5' |
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| 11520 | 5' | -55.8 | NC_003085.1 | + | 15760 | 0.66 | 0.673621 |
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Target: 5'- cGCUGCaaucGAgGCgUUUGAGCgcgucaacgcguuucUGGAGGCCa -3' miRNA: 3'- aCGACG----CUgCG-GAGCUUG---------------ACCUUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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