Results 81 - 85 of 85 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11520 | 5' | -55.8 | NC_003085.1 | + | 47613 | 0.66 | 0.647123 |
Target: 5'- cGCUGCGcauCGCUcgCGGGCUcgucggucccGGuGGCCg -3' miRNA: 3'- aCGACGCu--GCGGa-GCUUGA----------CCuUCGG- -5' |
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11520 | 5' | -55.8 | NC_003085.1 | + | 47952 | 0.67 | 0.569876 |
Target: 5'- gGCUGCcucuUGCC-CGAGCgccGGAcgGGCCg -3' miRNA: 3'- aCGACGcu--GCGGaGCUUGa--CCU--UCGG- -5' |
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11520 | 5' | -55.8 | NC_003085.1 | + | 48220 | 0.69 | 0.464377 |
Target: 5'- aGC-GCGACGCCgc--GCUGGAggaggcAGCCc -3' miRNA: 3'- aCGaCGCUGCGGagcuUGACCU------UCGG- -5' |
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11520 | 5' | -55.8 | NC_003085.1 | + | 48511 | 0.66 | 0.647123 |
Target: 5'- aUGCUcGCGGCcucGCCguccaugCGGA--GGAAGCCa -3' miRNA: 3'- -ACGA-CGCUG---CGGa------GCUUgaCCUUCGG- -5' |
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11520 | 5' | -55.8 | NC_003085.1 | + | 49294 | 0.71 | 0.378813 |
Target: 5'- cUGCUGCGuCGCgUCG-GCgucccgGGAGGCg -3' miRNA: 3'- -ACGACGCuGCGgAGCuUGa-----CCUUCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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