Results 81 - 85 of 85 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 11520 | 5' | -55.8 | NC_003085.1 | + | 47952 | 0.67 | 0.569876 |
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Target: 5'- gGCUGCcucuUGCC-CGAGCgccGGAcgGGCCg -3' miRNA: 3'- aCGACGcu--GCGGaGCUUGa--CCU--UCGG- -5' |
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| 11520 | 5' | -55.8 | NC_003085.1 | + | 41078 | 0.67 | 0.57973 |
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Target: 5'- gGCUGCcgaugagcgagcgGACuGCCUaccUGGACUGGGuggaGGCCc -3' miRNA: 3'- aCGACG-------------CUG-CGGA---GCUUGACCU----UCGG- -5' |
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| 11520 | 5' | -55.8 | NC_003085.1 | + | 39509 | 0.67 | 0.580827 |
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Target: 5'- aGCcgGUG-CGCCUCGucGCUGGcaucguccuGGCCg -3' miRNA: 3'- aCGa-CGCuGCGGAGCu-UGACCu--------UCGG- -5' |
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| 11520 | 5' | -55.8 | NC_003085.1 | + | 20346 | 0.67 | 0.580827 |
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Target: 5'- gGCcaCGGCGCCUC-AGCgcgGGAgcGGCCg -3' miRNA: 3'- aCGacGCUGCGGAGcUUGa--CCU--UCGG- -5' |
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| 11520 | 5' | -55.8 | NC_003085.1 | + | 10491 | 0.66 | 0.658182 |
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Target: 5'- cUGCUGCGcgGCGCaCUgGAGaauccgcGGAAGCg -3' miRNA: 3'- -ACGACGC--UGCG-GAgCUUga-----CCUUCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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