Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11523 | 3' | -48.8 | NC_003085.1 | + | 18814 | 0.66 | 0.968662 |
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Target: 5'- gCGGCGGCUUCGgcaugcugcgcaGGA--AGGCGCGUc- -3' miRNA: 3'- -GCUGUUGAAGC------------UCUcaUCUGCGCAag -5' |
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| 11523 | 3' | -48.8 | NC_003085.1 | + | 4508 | 0.66 | 0.962875 |
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Target: 5'- aCGGCGACggugaggagcaccgUCGggcaccGGAGUAGAUGCGcgCc -3' miRNA: 3'- -GCUGUUGa-------------AGC------UCUCAUCUGCGCaaG- -5' |
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| 11523 | 3' | -48.8 | NC_003085.1 | + | 17721 | 0.66 | 0.961319 |
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Target: 5'- aGGCAGa---GAGAG-AGGCGCGUg- -3' miRNA: 3'- gCUGUUgaagCUCUCaUCUGCGCAag -5' |
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| 11523 | 3' | -48.8 | NC_003085.1 | + | 28258 | 0.68 | 0.920403 |
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Target: 5'- gGACGGCUccaccUGGGAcGUGGACGUGUg- -3' miRNA: 3'- gCUGUUGAa----GCUCU-CAUCUGCGCAag -5' |
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| 11523 | 3' | -48.8 | NC_003085.1 | + | 915 | 0.68 | 0.907165 |
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Target: 5'- gGGCAGCgcgaCGuGAG-AGACGCGcUCg -3' miRNA: 3'- gCUGUUGaa--GCuCUCaUCUGCGCaAG- -5' |
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| 11523 | 3' | -48.8 | NC_003085.1 | + | 47022 | 0.7 | 0.842691 |
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Target: 5'- aCGACGGCgcgUUGcAGGGUAGggcaGCGCGUcuUCg -3' miRNA: 3'- -GCUGUUGa--AGC-UCUCAUC----UGCGCA--AG- -5' |
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| 11523 | 3' | -48.8 | NC_003085.1 | + | 10830 | 0.7 | 0.814274 |
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Target: 5'- gGGCAGCgacugCGGGAGUgGGAUGCGa-- -3' miRNA: 3'- gCUGUUGaa---GCUCUCA-UCUGCGCaag -5' |
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| 11523 | 3' | -48.8 | NC_003085.1 | + | 184 | 0.75 | 0.593084 |
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Target: 5'- gGGCAGCaucUCG-GAGUGGACGCGg-- -3' miRNA: 3'- gCUGUUGa--AGCuCUCAUCUGCGCaag -5' |
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| 11523 | 3' | -48.8 | NC_003085.1 | + | 4376 | 1.11 | 0.003599 |
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Target: 5'- aCGACAACUUCGAGAGUAGACGCGUUCc -3' miRNA: 3'- -GCUGUUGAAGCUCUCAUCUGCGCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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