Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
11523 | 5' | -59.8 | NC_003085.1 | + | 47042 | 0.73 | 0.176474 |
Target: 5'- aGGGCaGCGCGUCUucgucaccaccaGCUCggCGGUGUCCa- -3' miRNA: 3'- -CCCG-CGCGUAGA------------UGAG--GCCACGGGcu -5' |
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11523 | 5' | -59.8 | NC_003085.1 | + | 11129 | 0.73 | 0.171873 |
Target: 5'- gGGGCGCGCGUCguCUCuCGGaUGUgCCGAc -3' miRNA: 3'- -CCCGCGCGUAGauGAG-GCC-ACG-GGCU- -5' |
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11523 | 5' | -59.8 | NC_003085.1 | + | 13655 | 0.75 | 0.127946 |
Target: 5'- uGGCGCGC-----CUCCGGUGCCCa- -3' miRNA: 3'- cCCGCGCGuagauGAGGCCACGGGcu -5' |
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11523 | 5' | -59.8 | NC_003085.1 | + | 4411 | 1.1 | 0.000303 |
Target: 5'- uGGGCGCGCAUCUACUCCGGUGCCCGAc -3' miRNA: 3'- -CCCGCGCGUAGAUGAGGCCACGGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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