Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11524 | 3' | -55.3 | NC_003085.1 | + | 39511 | 0.66 | 0.679935 |
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Target: 5'- uGGCGGGCaaucauGGUGCgggaaUCGCcugCAGGCUGg -3' miRNA: 3'- -CUGCCUGcu----CCACG-----AGUGa--GUCUGAC- -5' |
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| 11524 | 3' | -55.3 | NC_003085.1 | + | 12581 | 0.66 | 0.668887 |
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Target: 5'- uGGCGGugGAGGcugcuguagUGCUCcCUCuGAUUc -3' miRNA: 3'- -CUGCCugCUCC---------ACGAGuGAGuCUGAc -5' |
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| 11524 | 3' | -55.3 | NC_003085.1 | + | 22516 | 0.66 | 0.668887 |
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Target: 5'- aGACGGGCu-GGcGCUC-CUCgacGGACUGg -3' miRNA: 3'- -CUGCCUGcuCCaCGAGuGAG---UCUGAC- -5' |
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| 11524 | 3' | -55.3 | NC_003085.1 | + | 21693 | 0.67 | 0.591162 |
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Target: 5'- --aGGACGAGGUGgcccgcUUCGcCUCGGACa- -3' miRNA: 3'- cugCCUGCUCCAC------GAGU-GAGUCUGac -5' |
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| 11524 | 3' | -55.3 | NC_003085.1 | + | 19243 | 0.67 | 0.576819 |
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Target: 5'- cGACGGcacacacaugcacuGCGAGGUGCUCuuccgagcGCUC-GACa- -3' miRNA: 3'- -CUGCC--------------UGCUCCACGAG--------UGAGuCUGac -5' |
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| 11524 | 3' | -55.3 | NC_003085.1 | + | 41207 | 0.68 | 0.547304 |
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Target: 5'- -cCGGccagcaguACGGGGUGCcgCGCUCGGcCUGg -3' miRNA: 3'- cuGCC--------UGCUCCACGa-GUGAGUCuGAC- -5' |
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| 11524 | 3' | -55.3 | NC_003085.1 | + | 21264 | 0.68 | 0.536489 |
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Target: 5'- cGACGGACaagguGGUGCcgCGCcCAGACUu -3' miRNA: 3'- -CUGCCUGcu---CCACGa-GUGaGUCUGAc -5' |
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| 11524 | 3' | -55.3 | NC_003085.1 | + | 31685 | 0.71 | 0.359921 |
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Target: 5'- -uCGGGCGGGGgcccuugGCuUCGCUCAGGgUGg -3' miRNA: 3'- cuGCCUGCUCCa------CG-AGUGAGUCUgAC- -5' |
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| 11524 | 3' | -55.3 | NC_003085.1 | + | 4976 | 1 | 0.00398 |
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Target: 5'- aGACGGACGAGGUGC-CACUCAGACUGa -3' miRNA: 3'- -CUGCCUGCUCCACGaGUGAGUCUGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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