Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11535 | 5' | -52.6 | NC_003085.1 | + | 47015 | 0.66 | 0.826041 |
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Target: 5'- cUCAGAGAcgacggcgCGUUGCAGGguagGGCaGCGc -3' miRNA: 3'- -GGUCUUUaa------GCAGCGUCCa---CCG-CGUa -5' |
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| 11535 | 5' | -52.6 | NC_003085.1 | + | 38144 | 0.66 | 0.804851 |
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Target: 5'- cCCugcugg-CGUUGguGGUGGCGCu- -3' miRNA: 3'- -GGucuuuaaGCAGCguCCACCGCGua -5' |
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| 11535 | 5' | -52.6 | NC_003085.1 | + | 10405 | 0.67 | 0.744844 |
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Target: 5'- cCCGGGAGgcgaCGUgGCGGG-GGcCGCAUc -3' miRNA: 3'- -GGUCUUUaa--GCAgCGUCCaCC-GCGUA- -5' |
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| 11535 | 5' | -52.6 | NC_003085.1 | + | 22998 | 0.68 | 0.689738 |
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Target: 5'- gCCAGAGGUgcgcgCGcCGCAGuccUGGCGCc- -3' miRNA: 3'- -GGUCUUUAa----GCaGCGUCc--ACCGCGua -5' |
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| 11535 | 5' | -52.6 | NC_003085.1 | + | 48723 | 0.68 | 0.689738 |
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Target: 5'- aCCGGAAGgacccaGUCgGCcuGUGGCGCAa -3' miRNA: 3'- -GGUCUUUaag---CAG-CGucCACCGCGUa -5' |
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| 11535 | 5' | -52.6 | NC_003085.1 | + | 42593 | 0.68 | 0.67849 |
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Target: 5'- gCGGGAGgcca-CGCAGGUGGCGUg- -3' miRNA: 3'- gGUCUUUaagcaGCGUCCACCGCGua -5' |
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| 11535 | 5' | -52.6 | NC_003085.1 | + | 23145 | 0.69 | 0.667195 |
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Target: 5'- gCAGAGcUUCGa-GCAGGUGGUGUc- -3' miRNA: 3'- gGUCUUuAAGCagCGUCCACCGCGua -5' |
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| 11535 | 5' | -52.6 | NC_003085.1 | + | 16646 | 0.7 | 0.576513 |
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Target: 5'- cCCGGG---UCGUCGCAGuGgccgGGCGCc- -3' miRNA: 3'- -GGUCUuuaAGCAGCGUC-Ca---CCGCGua -5' |
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| 11535 | 5' | -52.6 | NC_003085.1 | + | 20231 | 0.71 | 0.510231 |
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Target: 5'- uCCGGua---CGUCGCcaccuucgcccAGGUGGCGCAg -3' miRNA: 3'- -GGUCuuuaaGCAGCG-----------UCCACCGCGUa -5' |
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| 11535 | 5' | -52.6 | NC_003085.1 | + | 10224 | 1.07 | 0.002011 |
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Target: 5'- gCCAGAAAUUCGUCGCAGGUGGCGCAUu -3' miRNA: 3'- -GGUCUUUAAGCAGCGUCCACCGCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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