Results 1 - 3 of 3 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1155 | 5' | -54.7 | NC_001132.2 | + | 10963 | 0.76 | 0.502638 |
Target: 5'- cGGACGACUUg--GGAGAGaUCGUGCGGg -3' miRNA: 3'- -CUUGCUGAGaggCCUCUC-AGCACGCU- -5' |
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1155 | 5' | -54.7 | NC_001132.2 | + | 157704 | 0.84 | 0.181676 |
Target: 5'- -cACGACUCUCCGGAGAGUCGUu--- -3' miRNA: 3'- cuUGCUGAGAGGCCUCUCAGCAcgcu -5' |
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1155 | 5' | -54.7 | NC_001132.2 | + | 4254 | 1.09 | 0.004603 |
Target: 5'- gGAACGACUCUCCGGAGAGUCGUGCGAa -3' miRNA: 3'- -CUUGCUGAGAGGCCUCUCAGCACGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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