Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11556 | 3' | -57.2 | NC_003085.1 | + | 12241 | 0.7 | 0.342035 |
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Target: 5'- gCUGGgccCAUGCGGaacgaguucaUGCGCgACCGGUGc -3' miRNA: 3'- -GACCaa-GUACGCCg---------AUGCG-UGGCCGC- -5' |
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| 11556 | 3' | -57.2 | NC_003085.1 | + | 30329 | 0.71 | 0.310332 |
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Target: 5'- cCUGGggCGgcgGgGGCUGgGCGCCGcGCa -3' miRNA: 3'- -GACCaaGUa--CgCCGAUgCGUGGC-CGc -5' |
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| 11556 | 3' | -57.2 | NC_003085.1 | + | 34637 | 0.71 | 0.295339 |
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Target: 5'- cCUGGcu-GUGCGGCgcacGCGgGCCGGCu -3' miRNA: 3'- -GACCaagUACGCCGa---UGCgUGGCCGc -5' |
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| 11556 | 3' | -57.2 | NC_003085.1 | + | 23446 | 0.72 | 0.280209 |
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Target: 5'- uUGGUUgGggucgaagugcUGgGGCUGCGCcucggcgGCCGGCGg -3' miRNA: 3'- gACCAAgU-----------ACgCCGAUGCG-------UGGCCGC- -5' |
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| 11556 | 3' | -57.2 | NC_003085.1 | + | 1594 | 0.74 | 0.205937 |
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Target: 5'- gCUGGagUCAcgccugGCGGCcgugagGCGCACCGGCc -3' miRNA: 3'- -GACCa-AGUa-----CGCCGa-----UGCGUGGCCGc -5' |
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| 11556 | 3' | -57.2 | NC_003085.1 | + | 19788 | 1.09 | 0.000542 |
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Target: 5'- uCUGGUUCAUGCGGCUACGCACCGGCGg -3' miRNA: 3'- -GACCAAGUACGCCGAUGCGUGGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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