Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11557 | 5' | -56.5 | NC_003085.1 | + | 27813 | 0.66 | 0.556234 |
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Target: 5'- uCGCUCC-CGG-GCUGGAGUAugcaacUCCa -3' miRNA: 3'- -GCGAGGaGUCaCGACCUCGUca----AGGa -5' |
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| 11557 | 5' | -56.5 | NC_003085.1 | + | 3080 | 0.67 | 0.513167 |
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Target: 5'- gGCgUCgUCcGUGCgcccagcggGGAGCGGUUCCc -3' miRNA: 3'- gCG-AGgAGuCACGa--------CCUCGUCAAGGa -5' |
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| 11557 | 5' | -56.5 | NC_003085.1 | + | 48378 | 0.69 | 0.431674 |
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Target: 5'- gCGCUCCUgAGcGCgGcGAGCAGcgCCg -3' miRNA: 3'- -GCGAGGAgUCaCGaC-CUCGUCaaGGa -5' |
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| 11557 | 5' | -56.5 | NC_003085.1 | + | 23470 | 0.7 | 0.366942 |
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Target: 5'- uCGCUgCUCAGccUGCUGGAGgAGUUg-- -3' miRNA: 3'- -GCGAgGAGUC--ACGACCUCgUCAAgga -5' |
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| 11557 | 5' | -56.5 | NC_003085.1 | + | 49198 | 0.77 | 0.120385 |
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Target: 5'- uGCUCCUCAGUGagagGGGGagGGUUCCUc -3' miRNA: 3'- gCGAGGAGUCACga--CCUCg-UCAAGGA- -5' |
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| 11557 | 5' | -56.5 | NC_003085.1 | + | 19960 | 1.07 | 0.000844 |
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Target: 5'- gGCUCCUCAGUGCUGGAGCAGUUCCUg -3' miRNA: 3'- gCGAGGAGUCACGACCUCGUCAAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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