Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11558 | 5' | -56.4 | NC_003085.1 | + | 6687 | 0.66 | 0.550678 |
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Target: 5'- aUgGCCCUGGAgcGGCUUgcGGCCGCc -3' miRNA: 3'- aAgUGGGACCUacUUGAGacCCGGUG- -5' |
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| 11558 | 5' | -56.4 | NC_003085.1 | + | 20830 | 0.66 | 0.544098 |
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Target: 5'- -cCAUCCUGGAUGAggcgaccggcgccgaGCUCgccgcugcGGuGCCGCc -3' miRNA: 3'- aaGUGGGACCUACU---------------UGAGa-------CC-CGGUG- -5' |
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| 11558 | 5' | -56.4 | NC_003085.1 | + | 32454 | 0.67 | 0.528848 |
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Target: 5'- -aCGCCCgcggccucaUGGAUGAGCUaCUGgccgaGGCCAa -3' miRNA: 3'- aaGUGGG---------ACCUACUUGA-GAC-----CCGGUg -5' |
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| 11558 | 5' | -56.4 | NC_003085.1 | + | 41080 | 0.67 | 0.518052 |
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Target: 5'- -aCGCCCacgGcGAUGAcgACgccgCUGGGCCAg -3' miRNA: 3'- aaGUGGGa--C-CUACU--UGa---GACCCGGUg -5' |
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| 11558 | 5' | -56.4 | NC_003085.1 | + | 25144 | 0.67 | 0.486221 |
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Target: 5'- -aCACCCUucagguaGAUGAACUC--GGCCGCg -3' miRNA: 3'- aaGUGGGAc------CUACUUGAGacCCGGUG- -5' |
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| 11558 | 5' | -56.4 | NC_003085.1 | + | 45990 | 0.67 | 0.486221 |
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Target: 5'- --gACUCUGGcUGccuCUCUGGGCgCACa -3' miRNA: 3'- aagUGGGACCuACuu-GAGACCCG-GUG- -5' |
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| 11558 | 5' | -56.4 | NC_003085.1 | + | 5869 | 0.68 | 0.465524 |
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Target: 5'- gUCAaccCCUUGGGgaAGCUCUGGGUgACg -3' miRNA: 3'- aAGU---GGGACCUacUUGAGACCCGgUG- -5' |
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| 11558 | 5' | -56.4 | NC_003085.1 | + | 46535 | 0.71 | 0.303278 |
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Target: 5'- -cCGCCCUugGuGAUGGACUCUGGGUg-- -3' miRNA: 3'- aaGUGGGA--C-CUACUUGAGACCCGgug -5' |
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| 11558 | 5' | -56.4 | NC_003085.1 | + | 20325 | 1.07 | 0.000646 |
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Target: 5'- cUUCACCCUGGAUGAACUCUGGGCCACg -3' miRNA: 3'- -AAGUGGGACCUACUUGAGACCCGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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