Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 11565 | 5' | -53.7 | NC_003085.1 | + | 3179 | 0.66 | 0.779346 |
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Target: 5'- aGCCaGAAGUagccgUCCUCUC-GCGGGCc-- -3' miRNA: 3'- -CGGaCUUCA-----AGGAGAGgUGCCUGcac -5' |
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| 11565 | 5' | -53.7 | NC_003085.1 | + | 29490 | 0.66 | 0.779346 |
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Target: 5'- cGCCUGAg---CCUCcgCCAUGGGC-UGg -3' miRNA: 3'- -CGGACUucaaGGAGa-GGUGCCUGcAC- -5' |
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| 11565 | 5' | -53.7 | NC_003085.1 | + | 5049 | 0.66 | 0.766072 |
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Target: 5'- aGUCUG-AGUgagcaccucguccgUCUUCUgCGCGGugGUGc -3' miRNA: 3'- -CGGACuUCA--------------AGGAGAgGUGCCugCAC- -5' |
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| 11565 | 5' | -53.7 | NC_003085.1 | + | 13385 | 0.66 | 0.758824 |
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Target: 5'- cGUCaaGAAGUUCgUCggCCACGGACaUGa -3' miRNA: 3'- -CGGa-CUUCAAGgAGa-GGUGCCUGcAC- -5' |
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| 11565 | 5' | -53.7 | NC_003085.1 | + | 25083 | 0.67 | 0.748357 |
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Target: 5'- aCCUGAAGggUgUCgccagCACGGugGUGg -3' miRNA: 3'- cGGACUUCaaGgAGag---GUGCCugCAC- -5' |
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| 11565 | 5' | -53.7 | NC_003085.1 | + | 40937 | 0.68 | 0.683429 |
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Target: 5'- cGCCgaGAGGUgCCUCUCC-CaGGCGUc -3' miRNA: 3'- -CGGa-CUUCAaGGAGAGGuGcCUGCAc -5' |
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| 11565 | 5' | -53.7 | NC_003085.1 | + | 6610 | 0.68 | 0.650127 |
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Target: 5'- aGCagaUGggGgUCCUCaagUCCGgcgcCGGACGUGa -3' miRNA: 3'- -CGg--ACuuCaAGGAG---AGGU----GCCUGCAC- -5' |
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| 11565 | 5' | -53.7 | NC_003085.1 | + | 34486 | 0.7 | 0.53951 |
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Target: 5'- cCCUGggGcgCCUCccagggcaCCACGGACGc- -3' miRNA: 3'- cGGACuuCaaGGAGa-------GGUGCCUGCac -5' |
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| 11565 | 5' | -53.7 | NC_003085.1 | + | 23606 | 0.7 | 0.518008 |
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Target: 5'- cCCUGGAGgagcaUCUgcauUCUUCACGGACGUc -3' miRNA: 3'- cGGACUUCa----AGG----AGAGGUGCCUGCAc -5' |
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| 11565 | 5' | -53.7 | NC_003085.1 | + | 24674 | 1.12 | 0.000896 |
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Target: 5'- aGCCUGAAGUUCCUCUCCACGGACGUGg -3' miRNA: 3'- -CGGACUUCAAGGAGAGGUGCCUGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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