Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1157 | 5' | -53.1 | NC_001132.2 | + | 7150 | 1.09 | 0.00623 |
Target: 5'- gCGGCGUAAACGGAUGCGUGUACGCCAu -3' miRNA: 3'- -GCCGCAUUUGCCUACGCACAUGCGGU- -5' |
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1157 | 5' | -53.1 | NC_001132.2 | + | 160086 | 0.7 | 0.870827 |
Target: 5'- gGGUGagggaaagAGACGGAgGCGUGUACcCCAa -3' miRNA: 3'- gCCGCa-------UUUGCCUaCGCACAUGcGGU- -5' |
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1157 | 5' | -53.1 | NC_001132.2 | + | 6548 | 0.68 | 0.94179 |
Target: 5'- cCGGaCGaGGAUGGAucuauacggguaugUGuCGUGUACGCCu -3' miRNA: 3'- -GCC-GCaUUUGCCU--------------AC-GCACAUGCGGu -5' |
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1157 | 5' | -53.1 | NC_001132.2 | + | 58087 | 0.67 | 0.969545 |
Target: 5'- aCGGCGgaGAUGcAUGUGUuaucgugagugcGUACGCCGu -3' miRNA: 3'- -GCCGCauUUGCcUACGCA------------CAUGCGGU- -5' |
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1157 | 5' | -53.1 | NC_001132.2 | + | 2771 | 0.67 | 0.959608 |
Target: 5'- gCGuGCGUAuACGGggGCG-GUGCcCCGu -3' miRNA: 3'- -GC-CGCAUuUGCCuaCGCaCAUGcGGU- -5' |
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1157 | 5' | -53.1 | NC_001132.2 | + | 9144 | 0.67 | 0.955849 |
Target: 5'- aGGCGgugccGugGGGUGUGUGUACa--- -3' miRNA: 3'- gCCGCau---UugCCUACGCACAUGcggu -5' |
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1157 | 5' | -53.1 | NC_001132.2 | + | 120329 | 0.66 | 0.979679 |
Target: 5'- uCGGCcc-AACGGGUcguguuagacacgGuCGUGUACGCCu -3' miRNA: 3'- -GCCGcauUUGCCUA-------------C-GCACAUGCGGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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